LOCUS       sequence-138740-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Wong AS, Choi GC, Cui CH, Pregernig G, Milani P, Adam M, Perli SD, 
            Kazer SW, Gaillard A, Hermann M, Shalek AK, Fraenkel E, Lu TK
  TITLE     Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 10. pii: 201517883.
  PUBMED    26864203
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Feb 10. pii: 201517883."
COMMENT     Sequence Label: pAWp9-2
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(72..147)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(169..417)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(227..246)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(397..417)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(486..507)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     835..854
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atccagaggt tgattatcga taagcttgat atcgaattgt gcgtcaggaa ttccgttgcg
       61 gatccaaaaa agcaccgact cggtgccact ttttcaagtt gataacggac tagccttatt
      121 ttaacttgct atttctagct ctaaaactga tcttcatggt ctgggtgcgg tgtttcgtcc
      181 tttccacaag atatataaag ccaagaaatc gaaatacttt caagttacgg taagcatatg
      241 atagtccatt ttaaaacata attttaaaac tgcaaactac ccaagaaatt attactttct
      301 acgtcacgta ttttgtacta atatctttgt gtttacagtc aaattaattc taattatctc
      361 tctaacagcc ttgtatcgta tatgcaaata tgaaggaatc atgggaaata ggccctctag
      421 atcctagagt cgcggccgct ttacttgtac agctcgtcca tgccgagagt gatcccggcg
      481 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
      541 gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
      601 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
      661 ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg
      721 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
      781 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
      841 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgta
//
