LOCUS       sequence-138743-        1091 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-terminal tagging with mClover using CRIPSR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT
  TITLE     Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron 
            Tagging with Short Homology Donors.
  JOURNAL   Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001.
            Epub 2016 Mar 24.
  PUBMED    27052166
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.001"; journalName: "Cell Rep"; date: 
            "2016-04-5- 5"; volume: "15"; issue: "1"; pages: "210-8"
COMMENT     Sequence Label: pMK279 (mClover-Bsr)
FEATURES             Location/Qualifiers
     source          1..1091
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    102..223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(139..158)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     193..212
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    254..287
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        348..847
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(383..406)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     780..799
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
       61 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
      121 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
      181 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgtc gatcatattc
      241 aataaccctt aatataactt cgtataatgt atgctatacg aagttattag gtctgaagag
      301 gagtttacgt ccagccaagc ttaggatctc gacctcgaaa ttctaccggg taggggaggc
      361 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
      421 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccgactcc
      481 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
      541 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
      601 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
      661 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
      721 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
      781 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
      841 cctttcgacc tgcatccatc tagatctcga gcagctgaag cttaccgcta gcatgaaaac
      901 atttaacatt tctcaacaag atctagaatt agtagaagta gcgacagaga agattacaat
      961 gctttatgag gataataaac atcatgtggg agcggcaatt cgtacgaaaa caggagaaat
     1021 catttcggca gtacatattg aagcgtatat aggacgagta actgtttgtg cagaagccat
     1081 tgcgattggt a
//
