LOCUS       sequence-138747-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg1-U6p-GFP-sg1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Wong AS, Choi GC, Cui CH, Pregernig G, Milani P, Adam M, Perli SD, 
            Kazer SW, Gaillard A, Hermann M, Shalek AK, Fraenkel E, Lu TK
  TITLE     Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 10. pii: 201517883.
  PUBMED    26864203
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Feb 10. pii: 201517883."
COMMENT     Sequence Label: pAWp9-4
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             108..803
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat tgtccgctaa
       61 attctggccg tttttggctt ttttgttaga cagttaatta agccaccatg agcgagctga
      121 tcaaggagaa catgcacatg aagctgtaca tggagggcac cgtgaacaac caccacttca
      181 agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg aagatcaagg
      241 tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg
      301 gcagcaaagc cttcatcaac cacacccagg gcatccccga cttctttaag cagtccttcc
      361 ctgagggctt cacatgggag agaatcacca catacgaaga cgggggcgtg ctgaccgcta
      421 cccaggacac cagcttccag aacggctgca tcatctacaa cgtcaagatc aacggggtga
      481 acttcccatc caacggccct gtgatgcaga agaaaacacg cggctgggag gccaacaccg
      541 agatgctgta ccccgctgac ggcggcctga gaggccacag ccagatggcc ctgaagctcg
      601 tgggcggggg ctacctgcac tgctccttca agaccacata cagatccaag aaacccgcta
      661 agaacctcaa gatgcccggc ttccacttcg tggaccacag actggaaaga atcaaggagg
      721 ccgacaaaga gacctacgtc gagcagcacg agatggctgt ggccaagtac tgcgacctcc
      781 ctagcaaact ggggcacaga taataacctg caggcgggcc agatatacgc gttgacattg
      841 attattgact agttattaat agtaatcaat tacgg
//
