LOCUS       sequence-138749-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg1-U6p-GFP-sg1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Wong AS, Choi GC, Cui CH, Pregernig G, Milani P, Adam M, Perli SD, 
            Kazer SW, Gaillard A, Hermann M, Shalek AK, Fraenkel E, Lu TK
  TITLE     Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 10. pii: 201517883.
  PUBMED    26864203
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Feb 10. pii: 201517883."
COMMENT     Sequence Label: pAWp9-4
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(91..166)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(188..436)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        complement(450..525)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(547..795)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(864..885)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttgtaatcca gaggttgatt atcgataagc ttgatatcga attgtgcgtc aggaattgtg
       61 cgtcaggaat tccgttgcgg atccaaaaaa gcaccgactc ggtgccactt tttcaagttg
      121 ataacggact agccttattt taacttgcta tttctagctc taaaaccggt gaacagctcc
      181 tcgccccggt gtttcgtcct ttccacaaga tatataaagc caagaaatcg aaatactttc
      241 aagttacggt aagcatatga tagtccattt taaaacataa ttttaaaact gcaaactacc
      301 caagaaatta ttactttcta cgtcacgtat tttgtactaa tatctttgtg tttacagtca
      361 aattaattct aattatctct ctaacagcct tgtatcgtat atgcaaatat gaaggaatca
      421 tgggaaatag gccctctaga tccaaaaaag caccgactcg gtgccacttt ttcaagttga
      481 taacggacta gccttatttt aacttgctat ttctagctct aaaactgatc ttcatggtct
      541 gggtgcggtg tttcgtcctt tccacaagat atataaagcc aagaaatcga aatactttca
      601 agttacggta agcatatgat agtccatttt aaaacataat tttaaaactg caaactaccc
      661 aagaaattat tactttctac gtcacgtatt ttgtactaat atctttgtgt ttacagtcaa
      721 attaattcta attatctctc taacagcctt gtatcgtata tgcaaatatg aaggaatcat
      781 gggaaatagg ccctctagat cctagagtcg cggccgcttt acttgtacag ctcgtccatg
      841 ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg
      901 ttgg
//
