LOCUS       sequence-138750-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Wong AS, Choi GC, Cui CH, Pregernig G, Milani P, Adam M, Perli SD, 
            Kazer SW, Gaillard A, Hermann M, Shalek AK, Fraenkel E, Lu TK
  TITLE     Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 10. pii: 201517883.
  PUBMED    26864203
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Feb 10. pii: 201517883."
COMMENT     Sequence Label: pAWp9-5
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             109..804
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 aaatgtaatc atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta
       61 aattctggcc gtttttggct tttttgttag acagttaatt aagccaccat gagcgagctg
      121 atcaaggaga acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc
      181 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gaagatcaag
      241 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac
      301 ggcagcaaag ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc
      361 cctgagggct tcacatggga gagaatcacc acatacgaag acgggggcgt gctgaccgct
      421 acccaggaca ccagcttcca gaacggctgc atcatctaca acgtcaagat caacggggtg
      481 aacttcccat ccaacggccc tgtgatgcag aagaaaacac gcggctggga ggccaacacc
      541 gagatgctgt accccgctga cggcggcctg agaggccaca gccagatggc cctgaagctc
      601 gtgggcgggg gctacctgca ctgctccttc aagaccacat acagatccaa gaaacccgct
      661 aagaacctca agatgcccgg cttccacttc gtggaccaca gactggaaag aatcaaggag
      721 gccgacaaag agacctacgt cgagcagcac gagatggctg tggccaagta ctgcgacctc
      781 cctagcaaac tggggcacag ataataacct gcaggcgg
//
