LOCUS       sequence-138751-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Wong AS, Choi GC, Cui CH, Pregernig G, Milani P, Adam M, Perli SD, 
            Kazer SW, Gaillard A, Hermann M, Shalek AK, Fraenkel E, Lu TK
  TITLE     Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 10. pii: 201517883.
  PUBMED    26864203
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Feb 10. pii: 201517883."
COMMENT     Sequence Label: pAWp9-5
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(85..160)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(182..430)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        complement(444..519)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(541..789)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(858..879)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tccagaggtt gattatcgat aagcttgata tcgaattgtg cgtcaggaat tgtgcgtcag
       61 gaattccgtt gcggatccaa aaaagcaccg actcggtgcc actttttcaa gttgataacg
      121 gactagcctt attttaactt gctatttcta gctctaaaac cggtgaacag ctcctcgccc
      181 cggtgtttcg tcctttccac aagatatata aagccaagaa atcgaaatac tttcaagtta
      241 cggtaagcat atgatagtcc attttaaaac ataattttaa aactgcaaac tacccaagaa
      301 attattactt tctacgtcac gtattttgta ctaatatctt tgtgtttaca gtcaaattaa
      361 ttctaattat ctctctaaca gccttgtatc gtatatgcaa atatgaagga atcatgggaa
      421 ataggccctc tagatccaaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg
      481 actagcctta ttttaacttg ctatttctag ctctaaaacc ggatgtgcac ttgaagtggc
      541 ggtgtttcgt cctttccaca agatatataa agccaagaaa tcgaaatact ttcaagttac
      601 ggtaagcata tgatagtcca ttttaaaaca taattttaaa actgcaaact acccaagaaa
      661 ttattacttt ctacgtcacg tattttgtac taatatcttt gtgtttacag tcaaattaat
      721 tctaattatc tctctaacag ccttgtatcg tatatgcaaa tatgaaggaa tcatgggaaa
      781 taggccctct agatcctaga gtcgcggccg ctttacttgt acagctcgtc catgccgaga
      841 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgt
//
