LOCUS       sequence-138752-        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with 
            C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) 
            with C-terminal c-myc epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Kappock
  TITLE     Additional fatty acid desaturase and acetylenase constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK372
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(111..566)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(248..269)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     460..479
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(645..810)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     731..755
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(958..981)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgcatgtata ctaaactcac aaattagagc ttcaatttaa ttatatcagt tattacccta
       61 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag
      121 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
      181 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
      241 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
      301 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
      361 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
      421 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
      481 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
      541 gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa
      601 gggcgatcgg tgcgggcctc ttcgctatta cgccagctga attggagcga cctcatgcta
      661 tacctgagaa agcaacctga cctacaggaa agagttactc aagaataaga attttcgttt
      721 taaaacctaa gagtcacttt aaaatttgta tacacttatt ttttttataa cttatttaat
      781 aataaaaatc ataaatcata agaaattcgc ttatttagaa gtgtcaacaa cgtatctacc
      841 aacgatttga cccttttcca tcttttcgta aatttctggc aaggtagaca agccgacaac
      901 cttgattgga gacttgacca aacctctggc gaagaattgt taattaagag ctcagatctt
      961 atcgtcgtca tccttgtaat ccatcgatac tagtgcgaat ttattgtacc aatatacacc
     1021 cttggactcc ctacctttt
//
