LOCUS       sequence-138757-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 small hypothetical protein adjacent 
            to purEK genes and N5-carboxyaminoimidazole ribonucleotide 
            synthetase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK323
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             374..382
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggggatcctc tagagtcgac ctgcaggcat gctattcact ttgcgggccg ccacacccgg
       61 cgcacgattc cgcaccaatg gtgaaggaga caggacatca tgctgcgaga tcgcgattta
      121 ctgcttgccc aactgcatga actccggagt gaacacagag atctggatac ggttatcaac
      181 cgcatggcgc atgaaactac ttttattgac caactctatc tccagcggct gaaaaaacgt
      241 aaacttctgc taaaagatca gattaccaag gttgaaagcc ttctgatccc tgatgacatt
      301 gcctgaacct ctccgttaca gactttaaaa aaccggcctc tgtccccaac tctcccatta
      361 ccgaagataa atgacctcct tacattctcc gcttaagccc aatgccgtta ttggcattgt
      421 ggggggtggc cagcttggcc gcatgtctgc tgttgctgcc gcgcgccttg gttttcgcac
      481 gcatattctt acgccagagg ccaacggccc ggcggctcag gtctctcacg ccgtaacatg
      541 cggcaagtat gatgatccga cagcactgga tgcctttgcc cgcgctgttg atgttgttac
      601 ctttgaattt gaaaacatca gcgcagatgc actggaccgc ctagccaaac actgccctgt
      661 tcatccttcg gggcatattc tgcgcatcag tcaggaccga ttggcagaaa aaactttttt
      721 tgcttctgcg ggtattccac tcgcgccatg gcatcctgtt accgatgtca aaacgctaca
      781 cgaagccgca gaaaaagtgg gtttgccgct gattctcaaa accactcgca atggatatga
      841 tggcaaaggc cagcgccgtg tgcatcacat ccatgatctg gaagaggctt tcaacgctct
      901 ggcaccacat cctctggttg cagaaggtat ggtggatttt gcttgcgaga ttagtgttat
      961 ggtggcgcgt ggtgca
//
