LOCUS       sequence-138760-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-terminal tagging with mCherry2 using CRIPSR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT
  TITLE     Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron 
            Tagging with Short Homology Donors.
  JOURNAL   Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001.
            Epub 2016 Mar 24.
  PUBMED    27052166
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.001"; journalName: "Cell Rep"; date: 
            "2016-04-5- 5"; volume: "15"; issue: "1"; pages: "210-8"
COMMENT     Sequence Label: pMK281 (mCherry2-Hygro)
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(30..63)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gatcctagtg aacctcttcg agggacctaa taacttcgta tagcatacat tatacgaagt
       61 tatattaagg gttccggatc tcgaccagct tctgatggaa ttagaacttg gcaaaacaat
      121 actgagaatg aagtgtatgt ggaacagagg ctgctgatct cgttcttcag gctatgaaac
      181 tgacacattt ggaaaccaca gtacttagaa ccacaaagtg ggaatcaaga gaaaaacaat
      241 gatcccacga gagatctata gatctataga tcatgagtgg gaggaatgag ctggccctta
      301 atttggtttt gcttgtttaa attatgatat ccaactatga aacattatca taaagcaata
      361 gtaaagagcc ttcagtaaag agcaggcatt tatctaatcc caccccaccc ccacccccgt
      421 agctccaatc cttccattca aaatgtaggt actctgttct cacccttctt aacaaagtat
      481 gacaggaaaa acttccattt tagtggacat ctttattgtt taatagatca tcaatttctg
      541 catcccgggg atctgatatc atcgatgcat ggggtcgtgc gctcctttcg gtcgggcgct
      601 gcgggtcgtg gggcgggcgg ctagcctatt cctttgccct cggacgagtg ctggggcgtc
      661 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc
      721 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac
      781 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa
      841 gaccaatgcg gagcatatac gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc
      901 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt
      961 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta
     1021 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtg
//
