LOCUS       sequence-138773-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Production of Acetobacter aceti 1023 alanine racemase (alr) with 
            double mutant (AaAlr-K39A,Y260A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Francois JA, Kappock TJ
  TITLE     Alanine racemase from the acidophile Acetobacter aceti.
  JOURNAL   Protein Expr Purif. 2007 Jan;51(1):39-48. Epub 2006 Jun 6.
  PUBMED    16843006
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2007-01"; volume: "51(1)"; pages: "39-48. Epub 
            2006 Jun 6"
COMMENT     Sequence Label: pJK290
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatggctagc atgactggtg gacagcaagg
       61 gtcgcggatc cgaattcaat gtggccagca tcgcgcgcag gggctgtgtt gacgataaat
      121 ctttccgcta ttgccgaaaa ttaccgctat cttgtgaaca aactgggcaa aacagtttgc
      181 gccgcagtag ttgcggccga tgcttatggg cttggggttg cacaggtggc gcccattttg
      241 gaacaggttg gcgcaaaaca tttttttgtc gcacatgtgg atgaagggct agcccttagg
      301 ccatatcttt caccccacac atcagtaact gtgttgcatg gtccacggcc ggatgctgct
      361 gaggtttgcg tacgcaataa tttgcggcca gttttaaata gcctaaaaca ggtggctgtt
      421 ttgcgggatc tggcagccaa aatggataag ccgctggatg tggccattca actggatacg
      481 ggtatgtctc gttttggcct ttcagaggct gatatccata aactgatggc cactcctgaa
      541 cttttggagg ggttatctgt cagtctggtt atgagtcatc tggcctgtgc agatacgcca
      601 gaaaacgcag ccaatgccat gcagcgtgaa cggtttgaga actatgcacg catgttgcca
      661 aaggcagctt taagtctttc tgcatcttct ggaatttttc tggggcaacc atttcatttt
      721 gatctcgtgc gccctggtgc cgctttgtat ggcgtagcgc cgcagaatga tggtgttaat
      781 ccacttaagc ctgttgtggg gttgcaaacg catattctgc aaattcgcac tttatccaaa
      841 ggggatcggg tgggagctgg gcttacgtgg caagcgactg gcccatgccg tgttgcgacc
      901 attgcggttg gatatgctga tggatttgcc cgtgcgggcg ctgctgcagg atgcgcttgg
      961 tataaaggat atcggctacc aattctgggc cg
//
