LOCUS       sequence-138774-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Production of Acetobacter aceti 1023 alanine racemase (alr) with 
            double mutant (AaAlr-K39A,Y260A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Francois JA, Kappock TJ
  TITLE     Alanine racemase from the acidophile Acetobacter aceti.
  JOURNAL   Protein Expr Purif. 2007 Jan;51(1):39-48. Epub 2006 Jun 6.
  PUBMED    16843006
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2007-01"; volume: "51(1)"; pages: "39-48. Epub 
            2006 Jun 6"
COMMENT     Sequence Label: pJK290
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttc
       61 aggcagccgt tgcataaacg cgatgataac gccgccccag agatgtcagt acttcgtagc
      121 ctattgtgcc ttctgctttg gccacagcat ctacgccgta agactcattc aaaagttcca
      181 ccatgctatc ttcttccagc agcccttcgg gcactgcgga aagatcaact gtcatggaat
      241 ccatagagat tcggcccaga attggtagcc gatatccttt ataccaagcg catcctgcag
      301 cagcgcccgc acgggcaaat ccatcagcat atccaaccgc aatggtcgca acacggcatg
      361 ggccagtcgc ttgccacgta agcccagctc ccacccgatc ccctttggat aaagtgcgaa
      421 tttgcagaat atgcgtttgc aaccccacaa caggcttaag tggattaaca ccatcattct
      481 gcggcgctac gccatacaaa gcggcaccag ggcgcacgag atcaaaatga aatggttgcc
      541 ccagaaaaat tccagaagat gcagaaagac ttaaagctgc ctttggcaac atgcgtgcat
      601 agttctcaaa ccgttcacgc tgcatggcat tggctgcgtt ttctggcgta tctgcacagg
      661 ccagatgact cataaccaga ctgacagata acccctccaa aagttcagga gtggccatca
      721 gtttatggat atcagcctct gaaaggccaa aacgagacat acccgtatcc agttgaatgg
      781 ccacatccag cggcttatcc attttggctg ccagatcccg caaaacagcc acctgtttta
      841 ggctatttaa aactggccgc aaattattgc gtacgcaaac ctcagcagca tccggccgtg
      901 gaccatgcaa cacagttact gatgtgtggg gtgaaagata tggcctaagg gctagccctt
      961 catccacatg tgcgacaaaa aaatgttttg cgccaacctg ttccaaaatg ggcgcc
//
