LOCUS       sequence-138778-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses channelrhodopsin fused to YFP for monosynaptic circuit 
            mapping and optogenetic activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004."
COMMENT     Sequence Label: RabV CVS-N2c(deltaG)-hChR2-YFP
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(529..780)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(571..592)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tatgggtctc tggatgagaa gtgttgctag ttgttctttt ggtctacagt ttttttctcg
       61 actgaaatgc ttagatgacc cagccctcaa tagttatgta cacttcactg agatgcaaga
      121 tggttgtgaa ctattgctta tgtccatcat gggaggcaga ttgcctttct tagttgattc
      181 atgtcgagtc cgctatcggg gggcacacta cgacctcttt ctgtgcccaa gcacagagtc
      241 atccgaagta ccccttggcc tgtccacaac ctttggtatc agttcctgga gaatgaaagc
      301 accgttggtc actgaaactg ctagaagatc tcccgtatta agagaatcta cacgaccatg
      361 tctgatcaac tgaaatgatt aatgaaagtc ttatgactct tgaatctgcc ctgttggacg
      421 gagttcaagg aggactactg gatccaaaga ttccccccaa tcccgagagg aggttatcct
      481 cgggactgga atcgtcaaaa ggatgaccgg ctaggcgccg ctagcttact tgtacagctc
      541 gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg
      601 cttctcgttg gggtctttgc tcagggcgga ctggtagctc aggtagtggt tgtcgggcag
      661 cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct
      721 gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc
      781 ggccatgata tagacgttgt ggctgttgta gttgtactcc agct
//
