LOCUS       sequence-138781-        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Middle entry clone with photoconvertible eos conjugated to a nuclear
            localization signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Curran K, Lister JA, Kunkel GR, Prendergast A, Parichy DM, Raible DW
  TITLE     Interplay between Foxd3 and Mitf regulates cell fate plasticity in 
            the zebrafish neural crest.
  JOURNAL   Dev Biol. 2010 Aug 1;344(1):107-18. doi: 
            10.1016/j.ydbio.2010.04.023. Epub 2010 May 9.
  PUBMED    20460180
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2010.04.023"; journalName: "Dev Biol"; date: 
            "2010-08-1- 1"; volume: "344"; issue: "1"; pages: "107-18"
COMMENT     Sequence Label: pME nls-eos
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     161..177
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     161..177
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     182..201
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        182..200
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    218..317
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(324..1004)
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDITMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             complement(1011..1031)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gttggaattt aatcgcggcc tcgacgtttc ccgttgaata tggctcataa caccccttgt
       61 attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc
      121 aatgtaacat cagagatttt gagacacggg ccagagctgc caggaaacag ctatgaccat
      181 gtaatacgac tcactatagg ggatatcagc tggatggcaa ataatgattt tattttgact
      241 gatagtgacc tgttcgttgc aacaaattga taagcaatgc tttcttataa tgccaacttt
      301 gtacaagaaa gctgggtgga tccctatcgt ctggcattgt caggcaatcc agaatgagca
      361 acagcatgct catacagctt aaccttgttg taatctttgt catggcttaa aatctcaatg
      421 cagtggtcca caaagtggta gcctggtaac ttgacaccct tctccttagc tttgtaagta
      481 gttctgaagt cacatcggta atgggcattt ccttcaagca acaaagccat ggtaatatca
      541 cccgtcagca ctccatcacg cacatacatt ttctcagtgg agggctccca tttcagcgtc
      601 ttcttctgca taactggacc attggcggga aagtttacac cgtgaaatcg aactttatta
      661 tagaaagtgt ccccttccat tgttatgtcg tttctggcaa tgcaaatgcc cccgtcttcg
      721 aaagtcaagc ttcgttccca cgaatacccc ttaggaaacg actgcttaaa atagtcttgt
      781 atgtggtctg gatattcggc gaataccctg ttgccgtaat ggaatgcagt ggtcaggata
      841 tcaaaggcaa aaggcagagg tccgccctct ttgacttcaa gatccatact ctgttttccc
      901 tcaaaaggct tgcctgtacc atctccgtcg atcacaaagt ggtgcccgtt tacgttgcct
      961 tccatacgga ggttgatctt catgtctggc ttaatcgcac tcatgaattc taccttacgc
     1021 ttcttctttg gagccatgg
//
