LOCUS       sequence-138784-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Middle entry clone with photoconvertible eos conjugated to a nuclear
            localization signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Curran K, Lister JA, Kunkel GR, Prendergast A, Parichy DM, Raible DW
  TITLE     Interplay between Foxd3 and Mitf regulates cell fate plasticity in 
            the zebrafish neural crest.
  JOURNAL   Dev Biol. 2010 Aug 1;344(1):107-18. doi: 
            10.1016/j.ydbio.2010.04.023. Epub 2010 May 9.
  PUBMED    20460180
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2010.04.023"; journalName: "Dev Biol"; date: 
            "2010-08-1- 1"; volume: "344"; issue: "1"; pages: "107-18"
COMMENT     Sequence Label: pME nls-eos
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     210..227
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(386..413)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(505..591)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     640..662
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     654..671
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     655..671
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    687..786
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      789..798
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             801..821
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
      241 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
      301 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
      361 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
      421 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
      481 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
      541 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
      601 agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa
      661 cgacggccag tcttaagctc gggccccaaa taatgatttt attttgactg atagtgacct
      721 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
      781 caggctgcgc caccatggct ccaaagaaga agcgtaaggt agaattcatg agtgcgatta
      841 agccagacat gaagatcaac ctccgtatgg aaggcaacgt aa
//
