LOCUS       sequence-138785-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ratiometric pHluorin2 sequence in a middle entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Stawicki TM, Owens KN, Linbo T, Reinhart KE, Rubel EW, Raible DW
  TITLE     The zebrafish merovingian mutant reveals a role for pH regulation in
            hair cell toxicity and function.
  JOURNAL   Dis Model Mech. 2014 Jul;7(7):847-56. doi: 10.1242/dmm.016576.
  PUBMED    24973752
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dmm"; 
            journalName: "Dis Model Mech"; date: "2014-07"; volume: "7"; issue: 
            "7"; pages: "847-56"
COMMENT     Sequence Label: pME pHluorin2
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     155..171
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     155..171
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     176..195
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        176..194
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    212..311
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(370..391)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     719..738
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggtagcagcg aagtcgaggc
       61 atttctgtcc tggctggtct agaggaggca tgatgatata tttttatctt gtgcaatgta
      121 acatcagaga ttttgagaca cgggccagag ctgccaggaa acagctatga ccatgtaata
      181 cgactcacta taggggatat cagctggatg gcaaataatg attttatttt gactgatagt
      241 gacctgttcg ttgcaacaaa ttgatgagca attatttttt ataatgccaa ctttgtacaa
      301 gaaagctggg tcgaattcgc cctttcactt gtacagctcg tccatgccgt gagtgatccc
      361 ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct
      421 cagggcggac tgggtgtgca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat
      481 gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggtg
      541 cacctggaag atggccttgg tgccgttctt ctgcttgtcg gccatgatgt acaccaggtg
      601 ctcgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat
      661 gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt
      721 cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat
      781 ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac
      841 gccgtagctc agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca
      901 gatgaacttc agggtcagct tgc
//
