LOCUS       sequence-138792-        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Janthinobacterium lividum violacein biosynthesis protein 
            VioD with a N-terminal His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Kappock
  TITLE     Janthinobacterium lividum violacein biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK196
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgaaaattc tcgtcatcgg cgcaggcccc
      121 gcaggactgc tctttgccag tcaaatgaaa caggcccagc ccggctggga tatcagcatt
      181 acggaaaaaa acaccccgga agaagtgctg ggctggggcg tggtgctgcc ggggcggccg
      241 ccgcgccatc ccgccaatcc gctctcctac ctggagcagt ctgaacggct caatccgcag
      301 ttcctggaag aattcaagct cgtgcatcac gaccagccca acctgatgag caccggcgtt
      361 accctgtgcg gcgtcggacg ccaggccctg gtgcaggcac tgcgcgccaa gtgcgtggcg
      421 gccggcatcg ccatccgtta cgaaacgccg ccggcggaca aggcgcagct ggaagccgag
      481 tacgacctgg tggtggtatc gaatggcgtc aattacaaat cgctggagtt gccgccagca
      541 ctggcgccac acatcgattt cggccgcaac aaatacatct ggtacggcac cacccagctg
      601 ttcgaccaga tgaacctggt gttccgcagc aatgagcacg gcatattcat cggccatgcc
      661 tacaaatact cggacacgat gagcaccttt atcgtcgaat gcagcgaaga gacgtacgcc
      721 agggccgggc tggaggcgct gtccgagcgc gatgccgccg cgtacatcgc caaaacgttc
      781 aaggccgaac tcggtgagca tggactgcag agccaaccgg gccagggctg gcgcaacttc
      841 atgaccctca gccacgacca ggcctgcgac ggcaagttcg tcctgctcgg cgatgcgctg
      901 caatcggggc atttttccat cggccatggc accaccatgg cggtggtggt cgccctgctg
      961 ttggtcaaaa tcctcaatac cgaagacggc aaggccgccg cactggacag tttcaatgcg
     1021 cgtgccg
//
