LOCUS       sequence-138794-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PH domain for phosphoinositide binding 
            assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Yu JW, Mendrola JM, Audhya A, Singh S, Keleti D, DeWald DB, Murray 
            D, Emr SD, Lemmon MA
  TITLE     Genome-wide analysis of membrane targeting by S. cerevisiae 
            pleckstrin homology domains.
  JOURNAL   Mol Cell. 2004 Mar 12;13(5):677-88.
  PUBMED    15023338
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2004-03-12"; volume: "13(5)"; pages: "677-88"
COMMENT     Sequence Label: pGEX2TK-Osh2-PH
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(415..432)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(459..481)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gatcgtcagt cagtcacgat gaattcactt ttttttgttt aatatctcct tgtctttggc
       61 gaatctaata gcgctttgta tagcattgac ccacctagtc gtttcaatgg gatgattgcc
      121 ctttaagcgc caccttgttg tgcctgtgat gccacctagc aactcgaaat tcagtttttc
      181 tgaagaatcg atgtgtaagc ggcacgtcga aacttttaaa gtaccacgag gcctatcaac
      241 atgcgattgg tctttgtaat atgacagatt tccgtcgcca cttaaaatga accaacgtaa
      301 tttgtagcca tgggcaaaat ttgtccattt ctttaaaaac cccttatacg taggaggctt
      361 acttgatgcg acatcttcgt gaacaatagc ttgggatcca acagatgcac gacgagatcc
      421 acgcggaacc agatccgatt ttggaggatg gtcgccacca ccaaacgtgg cttgccagcc
      481 ctgcaaaggc catgctatat acttgctgga tttcaagtac ttatcaattt gtgggatagc
      541 ttcaatacgt tttttaaaac aaactaattt tgggaacgca tccaggcaca ttgggtccat
      601 gtataaaaca acatcaagag cgtcatacaa catgaagtca ggatgggtta catgatcacc
      661 atttaaatat gttttatgac ataaacgatc ttcgaacatt ttcagcattt caggtagctt
      721 gctaagaaaa tcaactttga gagtttcaaa gtctttacta tatgcaattc tcga
//
