LOCUS       sequence-138802-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For analysis of PH domain localization in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Yu JW, Mendrola JM, Audhya A, Singh S, Keleti D, DeWald DB, Murray 
            D, Emr SD, Lemmon MA
  TITLE     Genome-wide analysis of membrane targeting by S. cerevisiae 
            pleckstrin homology domains.
  JOURNAL   Mol Cell. 2004 Mar 12;13(5):677-88.
  PUBMED    15023338
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2004-03-12"; volume: "13(5)"; pages: "677-88"
COMMENT     Sequence Label: pRS426-GFP-Osh2-PH
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..47
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     31..47
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(573..601)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aagctggagc tccaccgcgg tggcggccgc tctagaacta gtggatcctt ctgcacaaga
       61 agccatattg acagagcagt atgtgaggac ctttcttcta cacaatgtaa cagtcaatgg
      121 ttgtagtcaa taccacgacc tttacgtgca gttttagagc gaaacttcgg ttttttgaga
      181 cttacctcgt gtttgtgtct ccctgggaat ggagccgcgc cacccttgtc tattatgtat
      241 agtcttattt aatcatagcg atgttggtca tccagtacac ttcggtagca accttcgttt
      301 gtgattgtct tggtaattgc ttccaacaac tttatccatc attgagacag gggccatatc
      361 acccgcgggg tctcaaagaa ggggcccact aataaaagca cgagataaga atgccagcaa
      421 aaaagctccg aaataattct tttcgtcttc cctcctagtc ttaacaagac aagagagaga
      481 gagagaaaga atactcacta gagattgttt cttttctact caacttaaag tatacatacg
      541 ctatgggtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      601 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
      661 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
      721 ttgtcactac ttttacgtat ggtgttcaat gcttttcaag atacccagat catatgaaac
      781 ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaa
//
