LOCUS       sequence-138804-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Neuronal-synaptobrevin (syb):spCFP1–10 chimera for synaptic GRASP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Macpherson LJ, Zaharieva EE, Kearney PJ, Alpert MH, Lin TY, Turan Z,
            Lee CH, Gallio M
  TITLE     Dynamic labelling of neural connections in multiple colours by 
            trans-synaptic fluorescence complementation.
  JOURNAL   Nat Commun. 2015 Dec 4;6:10024. doi: 10.1038/ncomms10024.
  PUBMED    26635273
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-12-4"; volume: "6:10024. doi"; pages: " 
            10.1038/ncomms10024"
COMMENT     Sequence Label: pQUAST-syb-spCFP1-10
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        251..489
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     436..457
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 ttaattagac gaaattattt ttaaagtttt atttttaata atttgcgagt acgcaaagct
       61 tggctgcatc caacgcgttg ggagctctcc ggatccaagc ttggatccgg gtaatcgctt
      121 atcctcggat aaacaattat cctcacgggt aatcgcttat ccgctcgggt aatcgcttat
      181 cctcgggtaa tcgcttatcc ttaagcttga tatcgaattg ctgcagcccg agcggagact
      241 ctagcgagcg ccggagtata aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa
      301 acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc gcagctgaac
      361 aagctaaaca atctgcagta aagtgcaagt taaagtgaat caattaaaag taaccagcaa
      421 ccaagtaaat caactgcaac tactgaaatc tgccaagaag taattattga atacaagaag
      481 agaactctga atagggaatt gggaattcgc tgaggccacc agagcaccac cagccaaacc
      541 acagaagcaa ccacacagcc accagagcca tggcggacgc tgcaccagct ggcgatgcac
      601 cacccaatgc cggagccccg gccggagagg gcggcgatgg cgagattgtg ggcggaccac
      661 acaatccgca gcagatcgcg gcacagaagc gtctgcagca gacgcaggcg caggtcgatg
      721 aggtcgtgga catcatgcgc acgaacgtgg agaaggtgct ggagcgcgac agcaagctgt
      781 cggagctgga cgaccgtgcc gatgccttgc agcagggtgc ctcgcagttt gagcagcagg
      841 cgggcaagct caagaggaaa ttctggcttc agaacttaaa gatgatgatc atcatgggcg
      901 tgattggcct ggttgtcgtg ggcattattg caaataaact tggactcata ggcggggagc
      961 agccgccaca gtaccagtat cccccacagt acatgcagcc accgccgccg ccaccgcagc
     1021 agccagccgg
//
