LOCUS       sequence-138811-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Neuronal-synaptobrevin (syb):spCFP1–10 chimera for synaptic GRASP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Macpherson LJ, Zaharieva EE, Kearney PJ, Alpert MH, Lin TY, Turan Z,
            Lee CH, Gallio M
  TITLE     Dynamic labelling of neural connections in multiple colours by 
            trans-synaptic fluorescence complementation.
  JOURNAL   Nat Commun. 2015 Dec 4;6:10024. doi: 10.1038/ncomms10024.
  PUBMED    26635273
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-12-4"; volume: "6:10024. doi"; pages: " 
            10.1038/ncomms10024"
COMMENT     Sequence Label: pQUAST-syb-spCFP1-10
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(114..755)
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFISDNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
ORIGIN
        1 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
       61 tgtcacacca cagaagtaag gttccttcac aaagatcctc tagactatgt tcctttttca
      121 tttggatctt tgctcaggac tgtttgtgtt gagaggtagt gattatcagg aagaagtacc
      181 ggaccgtctc caataggggt gttttgttgg taatggtcgg caagttgaac actgccatct
      241 tctacattat ggcgtactgt gaaattcgct ttaattccgt tcttttgctt atcagcagtg
      301 atgtatacgt tatcactaat aaaattgtat tcgagtttgt gtccaagaat attaccatct
      361 tctttaaaat ctgttccttt cagttcaatg cggttaacca aagtatctcc ttcaaattta
      421 acaactgctc tagttttata ttttccatca tctttgaacg aaattgttct ctcttgcacg
      481 taaccttcag gcatagccga cttaaaaaag tcatgtcttt tcatatggtc tgggtatctc
      541 gcaaagcact gaactcccca cgtaagcgtt gttacgagag tcggccatgg tacaggaagt
      601 ttaccagtag tacagatgaa tttaagagtc aattttccaa tggtggcgtc accttctcct
      661 tcgcctctca ctgagaactt atgtccgttg acatcaccat cgagttcaac caaaattggc
      721 acaacaccgg taaacagttc ttctcctttg gacattcctc gagccacgcc gccgtgatcg
      781 ccagctcctg ccgatcctcc tgctcctgct ccgtctcctg ctcctgccgc atccaccagc
      841 gatgactgtc ctccggctgg ctgctgcggt ggcggcggcg gtggctgcat gtactgtggg
      901 ggatactggt actgtggcgg ctgctccccg cctatgagtc caagtttatt tgcaataatg
      961 cccacgacaa ccaggccaat cacgcccatg atgatcatca tctttaagtt ctgaagccag
     1021 aatttcctct tgagcttgcc cgcctgctgc tcaaactgcg ag
//
