LOCUS       sequence-138812-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Phosphoinositide Binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Ferguson KM, Kavran JM, Sankaran VG, Fournier E, Isakoff SJ, Skolnik
            EY, Lemmon MA
  TITLE     Structural basis for discrimination of 3-phosphoinositides by 
            pleckstrin homology domains.
  JOURNAL   Mol Cell. 2000 Aug;6(2):373-84.
  PUBMED    10983984
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2000-08"; volume: "6(2)"; pages: "373-84"
COMMENT     Sequence Label: Dapp1-PH pET11a
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             399..421
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    436..460
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(460..479)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(461..479)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(563..582)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcagccggat ctatcggatc gtgccttccc cttggttgag ctgttttctt atttgtgaca
       61 atttccagcg taatatcttg atccactcat cagcttctac tcctgtcttt gcacagagat
      121 aaaatgtcct gaatggaaat accaaacaaa aacagtttac cctttcttgt gaataatcga
      181 attgtacagc tgaacattct gttaggtcta ggatccgaat tggttctggt gacatctggt
      241 ctttgaagta tttcagttca ttcctgtgca gagtaaacca tcttgttttc caggtcttga
      301 ccaggcctcc ctgtttggtg aggtaacctt ctttggtgcc cagagaaggt gctgtgggca
      361 caaggtcatc ttctgttctt cctgtctgca tatgtatatc tccttcttaa agttaaacaa
      421 aattatttct agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa
      481 tttcgcggga tcgagatctc gatcctctac gccggacgca tcgtggccgg catcaccggc
      541 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
      601 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
      661 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
      721 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
      781 gatcc
//
