LOCUS       sequence-138814-       10162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuronal-synaptobrevin (syb):spCFP1–10 chimera for synaptic GRASP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10162)
  AUTHORS   Macpherson LJ, Zaharieva EE, Kearney PJ, Alpert MH, Lin TY, Turan Z,
            Lee CH, Gallio M
  TITLE     Dynamic labelling of neural connections in multiple colours by 
            trans-synaptic fluorescence complementation.
  JOURNAL   Nat Commun. 2015 Dec 4;6:10024. doi: 10.1038/ncomms10024.
  PUBMED    26635273
REFERENCE   2  (bases 1 to 10162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-12-4"; volume: "6:10024. doi"; pages: " 
            10.1038/ncomms10024"
COMMENT     Sequence Label: pQUAST-syb-spCFP1-10
FEATURES             Location/Qualifiers
     source          1..10162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        3189..3427
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3374..3395
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             4066..4707
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFISDNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     intron          4800..4865
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4995..5015
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5287..5421
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5324..5343)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5378..5397
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     gene            5439..9566
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(5448..5466)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5917..5988,6383..6656,6731..7385,7447..7762,7983..8114,
                     8185..8799)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9577..10131,10132..10162)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgttggg agctctccgg atccaagctt ggatccgggt aatcgcttat
     3061 cctcggataa acaattatcc tcacgggtaa tcgcttatcc gctcgggtaa tcgcttatcc
     3121 tcgggtaatc gcttatcctt aagcttgata tcgaattgct gcagcccgag cggagactct
     3181 agcgagcgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac
     3241 aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa
     3301 gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta accagcaacc
     3361 aagtaaatca actgcaacta ctgaaatctg ccaagaagta attattgaat acaagaagag
     3421 aactctgaat agggaattgg gaattcgctg aggccaccag agcaccacca gccaaaccac
     3481 agaagcaacc acacagccac cagagccatg gcggacgctg caccagctgg cgatgcacca
     3541 cccaatgccg gagccccggc cggagagggc ggcgatggcg agattgtggg cggaccacac
     3601 aatccgcagc agatcgcggc acagaagcgt ctgcagcaga cgcaggcgca ggtcgatgag
     3661 gtcgtggaca tcatgcgcac gaacgtggag aaggtgctgg agcgcgacag caagctgtcg
     3721 gagctggacg accgtgccga tgccttgcag cagggtgcct cgcagtttga gcagcaggcg
     3781 ggcaagctca agaggaaatt ctggcttcag aacttaaaga tgatgatcat catgggcgtg
     3841 attggcctgg ttgtcgtggg cattattgca aataaacttg gactcatagg cggggagcag
     3901 ccgccacagt accagtatcc cccacagtac atgcagccac cgccgccgcc accgcagcag
     3961 ccagccggag gacagtcatc gctggtggat gcggcaggag caggagacgg agcaggagca
     4021 ggaggatcgg caggagctgg cgatcacggc ggcgtggctc gaggaatgtc caaaggagaa
     4081 gaactgttta ccggtgttgt gccaattttg gttgaactcg atggtgatgt caacggacat
     4141 aagttctcag tgagaggcga aggagaaggt gacgccacca ttggaaaatt gactcttaaa
     4201 ttcatctgta ctactggtaa acttcctgta ccatggccga ctctcgtaac aacgcttacg
     4261 tggggagttc agtgctttgc gagataccca gaccatatga aaagacatga cttttttaag
     4321 tcggctatgc ctgaaggtta cgtgcaagag agaacaattt cgttcaaaga tgatggaaaa
     4381 tataaaacta gagcagttgt taaatttgaa ggagatactt tggttaaccg cattgaactg
     4441 aaaggaacag attttaaaga agatggtaat attcttggac acaaactcga atacaatttt
     4501 attagtgata acgtatacat cactgctgat aagcaaaaga acggaattaa agcgaatttc
     4561 acagtacgcc ataatgtaga agatggcagt gttcaacttg ccgaccatta ccaacaaaac
     4621 acccctattg gagacggtcc ggtacttctt cctgataatc actacctctc aacacaaaca
     4681 gtcctgagca aagatccaaa tgaaaaagga acatagtcta gaggatcttt gtgaaggaac
     4741 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     4801 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     4861 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     4921 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     4981 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     5041 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     5101 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatatttga
     5161 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     5221 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     5281 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5341 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5401 gtatcttatc atgtctggat ccactagaag gcctaattct agtatgtatg taagttaata
     5461 aaacccattt ttgcggaaag tagataaaaa aaacattttt tttttttact gcactggata
     5521 tcattgaact tatctgatca gttttaaatt tacttcgatc caagggtatt tgatgtacca
     5581 ggttctttcg attacctctc actcaaaatg acattccact caaagtcagc gctgtttgcc
     5641 tccttctctg tccacagaaa tatcgccgtc tctttcgccg ctgcgtccgc tatctctttc
     5701 gccaccgttt gtagcgttac gtagcgtcaa tgtccgcctt cagttgcttt tgtcagcggt
     5761 ttcgtgacga agctccaagc ggtttacgcc atcaattaaa cacaaagtgc tgtgccaaaa
     5821 ctcctctcgc ttcttatttt tgtttgtttt ttgagtgatt ggggtggtga ttggttttgg
     5881 gtgggtaagc aggggaaagt gtgaaaaatc ccggcaatgg gccaagagga tcaggagcta
     5941 ttaattcgcg gaggcagcaa acacccatct gccgagcatc tgaacaatgt gagtagtaca
     6001 tgtgcataca tcttaagttc acttgatcta taggaactgc gattgcaaca tcaaattgtc
     6061 tgcggcgtga gaactgcgac ccacaaaaat cccaaaccgc aatcgcacaa acaaatagtg
     6121 acacgaaaca gattattctg gtagctgtgc tcgctatata agacaatttt taagatcata
     6181 tcatgatcaa gacatctaaa ggcattcatt ttcgactaca ttctttttta caaaaaatat
     6241 aacaaccaga tattttaagc tgatcctaga tgcacaaaaa ataaataaaa gtataaacct
     6301 acttcgtagg atacttcgtt ttgttcgggg ttagatgagc ataacgcttg tagttgatat
     6361 ttgagatccc ctatcattgc agggtgacag cggagcggct tcgcagagct gcattaacca
     6421 gggcttcggg caggccaaaa actacggcac gctcctgcca cccagtccgc cggaggactc
     6481 cggttcaggg agcggccaac tagccgagaa cctcacctat gcctggcaca atatggacat
     6541 ctttggggcg gtcaatcagc cgggctccgg atggcggcag ctggtcaacc ggacacgcgg
     6601 actattctgc aacgagcgac acataccggc gcccaggaaa catttgctca agaacggtga
     6661 gtttctattc gcagtcggct gatctgtgtg aaatcttaat aaagggtcca attaccaatt
     6721 tgaaactcag tttgcggcgt ggcctatccg ggcgaacttt tggccgtgat gggcagttcc
     6781 ggtgccggaa agacgaccct gctgaatgcc cttgcctttc gatcgccgca gggcatccaa
     6841 gtatcgccat ccgggatgcg actgctcaat ggccaacctg tggacgccaa ggagatgcag
     6901 gccaggtgcg cctatgtcca gcaggatgac ctctttatcg gctccctaac ggccagggaa
     6961 cacctgattt tccaggccat ggtgcggatg ccacgacatc tgacctatcg gcagcgagtg
     7021 gcccgcgtgg atcaggtgat ccaggagctt tcgctcagca aatgtcagca cacgatcatc
     7081 ggtgtgcccg gcagggtgaa aggtctgtcc ggcggagaaa ggaagcgtct ggcattcgcc
     7141 tccgaggcac taaccgatcc gccgcttctg atctgcgatg agcccacctc cggactggac
     7201 tcatttaccg cccacagcgt cgtccaggtg ctgaagaagc tgtcgcagaa gggcaagacc
     7261 gtcatcctga ccattcatca gccgtcttcc gagctgtttg agctctttga caagatcctt
     7321 ctgatggccg agggcagggt agctttcttg ggcactccca gcgaagccgt cgacttcttt
     7381 tcctagtgag ttcgatgtgt ttattaaggg tatctagcat tacattacat ctcaactcct
     7441 atccagcgtg ggtgcccagt gtcctaccaa ctacaatccg gcggactttt acgtacaggt
     7501 gttggccgtt gtgcccggac gggagatcga gtcccgtgat cggatcgcca agatatgcga
     7561 caattttgct attagcaaag tagcccggga tatggagcag ttgttggcca ccaaaaattt
     7621 ggagaagcca ctggagcagc cggagaatgg gtacacctac aaggccacct ggttcatgca
     7681 gttccgggcg gtcctgtggc gatcctggct gtcggtgctc aaggaaccac tcctcgtaaa
     7741 agtgcgactt attcagacaa cggtgagtgg ttccagtgga aacaaatgat ataacgctta
     7801 caattcttgg aaacaaattc gctagatttt agttagaatt gcctgattcc acacccttct
     7861 tagttttttt caatgagatg tatagtttat agttttgcag aaaataaata aatttcattt
     7921 aactcgcgaa catgttgaag atatgaatat taatgagatg cgagtaacat tttaatttgc
     7981 agatggttgc catcttgatt ggcctcatct ttttgggcca acaactcacg caagtgggcg
     8041 tgatgaatat caacggagcc atcttcctct tcctgaccaa catgaccttt caaaacgtct
     8101 ttgccacgat aaatgtaagt cttgtttaga atacatttgc atattaataa tttactaact
     8161 ttctaatgaa tcgattcgat ttaggtgttc acctcagagc tgccagtttt tatgagggag
     8221 gcccgaagtc gactttatcg ctgtgacaca tactttctgg gcaaaacgat tgccgaatta
     8281 ccgctttttc tcacagtgcc actggtcttc acggcgattg cctatccgat gatcggactg
     8341 cgggccggag tgctgcactt cttcaactgc ctggcgctgg tcactctggt ggccaatgtg
     8401 tcaacgtcct tcggatatct aatatcctgc gccagctcct cgacctcgat ggcgctgtct
     8461 gtgggtccgc cggttatcat accattcctg ctctttggcg gcttcttctt gaactcgggc
     8521 tcggtgccag tatacctcaa atggttgtcg tacctctcat ggttccgtta cgccaacgag
     8581 ggtctgctga ttaaccaatg ggcggacgtg gagccgggcg aaattagctg cacatcgtcg
     8641 aacaccacgt gccccagttc gggcaaggtc atcctggaga cgcttaactt ctccgccgcc
     8701 gatctgccgc tggactacgt gggtctggcc attctcatcg tgagcttccg ggtgctcgca
     8761 tatctggctc taagacttcg ggcccgacgc aaggagtagc cgacatatat ccgaaataac
     8821 tgcttgtttt tttttttacc attattacca tcgtgtttac tgtttattgc cccctcaaaa
     8881 agctaatgta attatatttg tgccaataaa aacaagatat gacctataga atacaagtat
     8941 ttccccttcg aacatcccca caagtagact ttggatttgt cttctaacca aaagacttac
     9001 acacctgcat accttacatc aaaaactcgt ttatcgctac ataaaacacc gggatatatt
     9061 ttttatatac atacttttca aatcgcgcgc cctcttcata attcacctcc accacaccac
     9121 gtttcgtagt tgctctttcg ctgtctccca cccgctctcc gcaacacatt caccttttgt
     9181 tcgacgacct tggagcgact gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc
     9241 gagtggttca tttcgtctca atagaaatta gtaataaata tttgtatgta caatttattt
     9301 gctccaatat atttgtatat atttccctca cagctatatt tattctaatt taatattatg
     9361 actttttaag gtaatttttt gtgacctgtt cggagtgatt agcgttacaa tttgaactga
     9421 aagtgacatc cagtgtttgt tccttgtgta gatgcatctc aaaaaaatgg tgggcataat
     9481 agtgttgttt atatatatca aaaataacaa ctataataat aagaatacat ttaatttaga
     9541 aaatgcttgg atttcactgg aactagaatt aattcggctg ctgctctaaa cgacgcattt
     9601 cgtactccaa agtacgaatt ttttccctca agctcttatt ttcattaaac aatgaacagg
     9661 acctaacgca cagtcacgtt attgtttaca taaatgattt tttttactat tcaaacttac
     9721 tctgtttgtg tactcccact ggtatagcct tcttttatct tttctggttc aggctctatc
     9781 actttactag gtacggcatc tgcgttgagt cgcctccttt taaatgtctg accttttgca
     9841 ggtgcagcct tccactgcga atcattaaag tgggtatcac aaatttggga gttttcacca
     9901 aggctgcacc caaggctctg ctcccacaat tttctcttaa tagcacactt cggcacgtga
     9961 attaatttta ctccagtcac agctttgcag caaaatttgc aatatttcat ttttttttat
    10021 tccacgtaag ggttaatgtt ttcaaaaaaa aattcgtccg cacacaacct ttcctctcaa
    10081 caagcaaacg tgcactgaat ttaagtgtat acttcggtaa gcttcggcta tcgacgggac
    10141 caccttatgt tatttcatca tg
//
