LOCUS       sequence-138815-         661 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Phosphoinositide Binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Ferguson KM, Kavran JM, Sankaran VG, Fournier E, Isakoff SJ, Skolnik
            EY, Lemmon MA
  TITLE     Structural basis for discrimination of 3-phosphoinositides by 
            pleckstrin homology domains.
  JOURNAL   Mol Cell. 2000 Aug;6(2):373-84.
  PUBMED    10983984
REFERENCE   2  (bases 1 to 661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2000-08"; volume: "6(2)"; pages: "373-84"
COMMENT     Sequence Label: Grp1-PH pET11a
FEATURES             Location/Qualifiers
     source          1..661
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             408..430
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    445..469
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(469..488)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(470..488)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(572..591)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cagccggatc ctacctcgtg gccaacatgt catagaacgg gtccctactg atgcttgctt
       61 tgatggactt catccactcc tccttttcct ccgggctggg ggcagagatc cggtacacaa
      121 cgtggttgcc ctccaccaca cggccatcgg cctccgtctt gcaggccttg atgacttgac
      181 ctttgtgact ggggttatac agctcaaagc agttcggctt ccgggggtcc tccacctccc
      241 tgatgctgag gttctccagg gggatgatgc ccctgggctc cttgtccgtg gtgtactcaa
      301 agtagtagag gcagttatct gtgaggatga accagcgccg tttccaggtc ttcacacgac
      361 cccccagctt cagcagccag ccttctcggt ctgggttcat atgtatatct ccttcttaaa
      421 gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag
      481 tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc
      541 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
      601 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
      661 c
//
