LOCUS       sequence-138817-         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PH domain for phosphoinositide binding 
            assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Kavran JM, Klein DE, Lee A, Falasca M, Isakoff SJ, Skolnik EY, 
            Lemmon MA
  TITLE     Specificity and promiscuity in phosphoinositide binding by 
            pleckstrin homology domains.
  JOURNAL   J Biol Chem. 1998 Nov 13;273(46):30497-508.
  PUBMED    9804818
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1998-11-13"; volume: "273(46)"; pages: "30497-508"
COMMENT     Sequence Label: Grp1-PH pGEX2TK
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(503..520)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(547..569)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 cagtcagtca cgatgaattc tatttcttat tggcaatcct ccttttcctc gtggccaaca
       61 tgtcatagaa cgggtcccta ctgatgcttg ctttgatgga cttcatccac tcctcctttt
      121 cctccgggct gggggcagag atccggtaca caacgtggtt gccctccacc acacggccat
      181 cggcctccgt cttgcaggcc ttgatgactt gacctttgtg actggggtta tacagctcaa
      241 agcagttcgg cttccggggg tcctccacct ccctgatgct gaggttctcc agggggatga
      301 tgcccctggg ctccttgtcc gtggtgtact caaagtagta gaggcagtta tctgtgagga
      361 tgaaccagcg ccgtttccag gtcttcacac gaccccccag cttcagcagc cagccttctc
      421 ggtctgggtt gaagaacgtg tgtgtcaggt catttccgtc gtcttctggg atcttaaacg
      481 gggatccaac agatgcacga cgagatccac gcggaaccag atccgatttt ggaggatggt
      541 cgccaccacc aaacgtggct tgccagccct gcaaaggcca tgctatatac ttgctggatt
      601 tcaagtactt atcaatttgt gggatagctt caatacgttt tttaaaacaa actaattttg
      661 ggaacgcatc caggcacatt gggtccatgt ataaaacaac atcaagagcg tcatacaaca
      721 tgaagtcagg atgg
//
