LOCUS       sequence-138818-        7298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the LRRC4C protein, 
            C-terminally fused to the Fc region of human IgG1 + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Lrrc4c-Fc-His
FEATURES             Location/Qualifiers
     source          1..7298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2775..2792
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2818..2939
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2855..2874)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2909..2928
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2977..2994)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3148..3736)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3228..3247)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3907..4698,4699..4767))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4530..4549
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4768..4872)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4940..4958
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4996..5018)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5020..5040)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5025..5355
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5206..5341
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5268..5287
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     5492..5511
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(5578..6033)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5715..5736)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5927..5946
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6159..6181
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6173..6190
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6174..6190
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        6220..6603
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6604..6807
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6758..6778
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6804..6828
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          join(6943..7298,1..470)
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
ORIGIN
        1 acatagcgtg ggatctccac gcgaatctcg ggtacgtgtt ccggacatgg gctcttctcc
       61 ggtagcggcg gagcttccac atccgagccc tggtcccatg cctccagcgg ctcatggtcg
      121 ctcggcagct ccttgctcct aacagtggag gccagactta ggcacagcac aatgcccacc
      181 accaccagtg tgccgcacaa ggccgtggcg gtagggtatg tgtctgaaaa tgagctcgga
      241 gattgggctc gcaccgctga cgcagatgga agacttaagg cagcggcaga agaagatgca
      301 ggcagctgag ttgttgtatt ctgataagag tcagaggtaa ctcccgttgc ggtgctgtta
      361 acggtggagg gcagtgtagt ctgagcagta ctcgttgctg ccgcgcgcgc caccagacat
      421 aatagctgac agactaacag actgttcctt tccatgggtc ttttctgcag aagcttgaat
      481 tctcgaggat ccgcggccgc gccaccatgt tgaacaagat gaccttacat ccacagcaga
      541 taatgatagg tcctaggttt aacagggccc tatttgaccc cctgcttgtg gtgctgttgg
      601 ctcttcaact tcttgtggtg gctggtttgg ttcgtgctca aacctgccct tcagtgtgct
      661 cttgtagcaa ccagttcagc aaggtgattt gtgttcggaa aaaccttcgt gaagttccgg
      721 atggcatctc caccaacaca aggctgctga acctccatga gaaccaaatc cagatcatta
      781 aagtgaacag cttcaagcat ttgaggcact tagaaatcct ccagttgagc aggaaccata
      841 ttcgaaccat tgaaatcggg gccttcaatg gtctggccaa cctcaacacc ctggaactct
      901 ttgacaatcg tcttactacc ataccgaatg gagcttttgt atatttgtct aaactgaagg
      961 agctctggtt gaggaacaac cctattgaaa gcatcccttc ctatgctttt aacagaatcc
     1021 cttctttgcg ccgcctggac ttaggggaat tgaaaaggct ttcatacatc tcagaaggtg
     1081 cctttgaagg tctgtccaac ttgaggtatt tgaaccttgc catgtgcaac cttcgggaaa
     1141 tccctaacct cacaccactc atcaaacttg acgagctaga tctttctggg aaccatttgt
     1201 ctgcaatcag gcctggctct tttcaggggt tgatgcacct tcaaaaactg tggatgatac
     1261 agtctcagat tcaagtgatt gaacggaatg cctttgataa ccttcagtca ctagtggaga
     1321 taaacttggc acacaacaat ctaacattat tgcctcatga cctcttcaca cccttgcatc
     1381 atctagagag gatacacctc catcacaacc cgtggaactg taactgtgat atcctgtggc
     1441 tcagctggtg gataagagac atggccccct ccaacacagc ttgctgtgcc aggtgtaaca
     1501 ctccccccaa cctgaaaggg aggtacatcg gagagctgga ccagaattac tttacatgct
     1561 atgctcccgt aattgtggag ccccctgcag acctcaatgt cactgaaggc atggcagctg
     1621 agctgaaatg tcgggcttct acgtccctga cttccgtatc ttggattact ccaaatggaa
     1681 cagtcatgac ccacggggca tacaaagtgc ggatagctgt gctcagcgac ggtactttaa
     1741 atttcacaaa tgtaactgtg caagacacag gcatgtacac atgtatggtg agtaattctg
     1801 ttggcaacac cactgcttct gccaccttga atgttactgc ggcaaccact actccgttct
     1861 cgtacttttc aactgtaaca gtagagacta tggaaccttc tcaggatgag gcacggacca
     1921 cagataacaa tgtgggcccc actccagtga tcgattggga gaccaccaat gtaaccacat
     1981 ctcttacgcc acagagcaca aggtcgacag aaaaaacatt caccatccca gtaactgaca
     2041 tcaacagcgg aatcccagga attgatgagg tcatgaaaac agctagtgac aaaactcaca
     2101 catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc ctcttccccc
     2161 caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc gtggtggtgg
     2221 acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc gtggaggtgc
     2281 ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt gtggtcagcg
     2341 tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc aaggtctcca
     2401 acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg cagccccgag
     2461 aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac caggtcagcc
     2521 tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg gagagcaatg
     2581 ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac ggctccttct
     2641 tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac gtcttctcat
     2701 gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc tccctgtctc
     2761 cgggtaaagg cggccatcat caccatcacc attaatctag agatcttatt aaagcagaac
     2821 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     2881 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     2941 catgtctggt cgactctagc tagactcttc cgcttcctcg ctcactgact cgctgcgctc
     3001 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3061 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3121 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3181 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3241 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3301 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3361 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3421 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3481 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3541 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     3601 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     3661 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     3721 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     3781 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     3841 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     3901 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     3961 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4021 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4081 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4141 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4201 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4261 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     4321 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     4381 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4441 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4501 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     4561 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4621 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4681 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     4741 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     4801 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     4861 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     4921 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     4981 tgatgacggt gaaaacctct gacacatgca gctcccggag gaatgtgtgt cagttagggt
     5041 gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc aaagcatgca tctcaattag
     5101 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5161 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     5221 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5281 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5341 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcgtccc ggagacggtc
     5401 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     5461 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     5521 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa
     5581 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     5641 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     5701 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     5761 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     5821 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     5881 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     5941 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     6001 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg
     6061 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     6121 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     6181 gacggccagt gaattgtcga ggtctcgacg gtatcgattg acattgatta ttgactagct
     6241 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     6301 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     6361 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     6421 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     6481 agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     6541 atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     6601 atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga
     6661 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     6721 gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta
     6781 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     6841 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggccgg
     6901 gaacggtgca ttggaacgcg gattccccgt gccaagagtg acgtaagtac cgcctataga
     6961 ctctataggc acaccccttt ggctcttatg catgctatac tgtttttggc ttggggccta
     7021 tacacccccg cttccatatg ctataggtga tggtatagct tagcctatag gtgtgggtta
     7081 ttgaccatta ttgaccactc ccctattggt gacgatactt tccattacta atccataaca
     7141 tggctctttg ccacaactat ctctattggc tatatgccaa tactctgtcc ttcagagact
     7201 gacacggact ctgtattttt acaggatggg gtcccattta ttatttacaa attcacatat
     7261 acaacaacgc cgtcccccgt gcccgcagtt tttattaa
//
