LOCUS       sequence-138820-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Production of Acetobacter aceti 1023 alanine racemase (alr) with 
            Ala367>Asp mutant (H6AaAlr-A367D).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Francois JA, Kappock TJ
  TITLE     Alanine racemase from the acidophile Acetobacter aceti.
  JOURNAL   Protein Expr Purif. 2007 Jan;51(1):39-48. Epub 2006 Jun 6.
  PUBMED    16843006
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2007-01"; volume: "51(1)"; pages: "39-48. Epub 
            2006 Jun 6"
COMMENT     Sequence Label: pJK303
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgtggcca gcatcgcgcg caggggctgt
       61 gttgacgata aatctttccg ctattgccga aaattaccgc tatcttgtga acaaactggg
      121 caaaacagtt tgcgccgcag tagttaaggc cgatgcttat gggcttgggg ttgcacaggt
      181 ggcgcccatt ttggaacagg ttggcgcaaa acattttttt gtcgcacatg tggatgaagg
      241 gctagccctt aggccatatc tttcacccca cacatcagta actgtgttgc atggtccacg
      301 gccggatgct gctgaggttt gcgtacgcaa taatttgcgg ccagttttaa atagcctaaa
      361 acaggtggct gttttgcggg atctggcagc caaaatggat aagccgctgg atgtggccat
      421 tcaactggat acgggtatgt ctcgttttgg cctttcagag gctgatatcc ataaactgat
      481 ggccactcct gaacttttgg aggggttatc tgtcagtctg gttatgagtc atctggcctg
      541 tgcagatacg ccagaaaacg cagccaatgc catgcagcgt gaacggtttg agaactatgc
      601 acgcatgttg ccaaaggcag ctttaagtct ttctgcatct tctggaattt ttctggggca
      661 accatttcat tttgatctcg tgcgccctgg tgccgctttg tatggcgtag cgccgcagaa
      721 tgatggtgtt aatccactta agcctgttgt ggggttgcaa acgcatattc tgcaaattcg
      781 cactttatcc aaaggggatc gggtgggata tgggcttacg tggcaagcga ctggcccatg
      841 ccgtgttgcg accattgcgg ttggatatgc tgatggattt gcccgtgcgg gcgctgctgc
      901 aggatgcgct tggtataaag gatatcggct accaattctg ggccgaatct ctatggattc
      961 catgacagtt gatctttccg cagtgcccga agggctgctg gaagaagata gcatggtgga
     1021 acttttgaat ga
//
