LOCUS       sequence-138823-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Neuronal-synaptobrevin (syb):spGFP1–10 chimera for synaptic GRASP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Macpherson LJ, Zaharieva EE, Kearney PJ, Alpert MH, Lin TY, Turan Z,
            Lee CH, Gallio M
  TITLE     Dynamic labelling of neural connections in multiple colours by 
            trans-synaptic fluorescence complementation.
  JOURNAL   Nat Commun. 2015 Dec 4;6:10024. doi: 10.1038/ncomms10024.
  PUBMED    26635273
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-12-4"; volume: "6:10024. doi"; pages: " 
            10.1038/ncomms10024"
COMMENT     Sequence Label: pUAS-syb:spGFP1–10
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    120..214
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        233..471
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     418..439
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 atattaatta gacgaaatta tttttaaagt tttattttta ataatttgcg agtacgcaaa
       61 gcttggctgc atccaacgcg ttgggagctc tccggatcca agcttgcatg cctgcaggtc
      121 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
      181 gtactgtcct ccgagcggag tactgtcctc cgagcggaga ctctagcgag cgccggagta
      241 taaatagagg cgcttcgtct acggagcgac aattcaattc aaacaagcaa agtgaacacg
      301 tcgctaagcg aaagctaagc aaataaacaa gcgcagctga acaagctaaa caatctgcag
      361 taaagtgcaa gttaaagtga atcaattaaa agtaaccagc aaccaagtaa atcaactgca
      421 actactgaaa tctgccaaga agtaattatt gaatacaaga agagaactct gaatagggaa
      481 ttgggaattc gctgaggcca ccagagcacc accagccaaa ccacagaagc aaccacacag
      541 ccaccagagc catggcggac gctgcaccag ctggcgatgc accacccaat gccggagccc
      601 cggccggaga gggcggcgat ggcgagattg tgggcggacc acacaatccg cagcagatcg
      661 cggcacagaa gcgtctgcag cagacgcagg cgcaggtcga tgaggtcgtg gacatcatgc
      721 gcacgaacgt ggagaaggtg ctggagcgcg acagcaagct gtcggagctg gacgaccgtg
      781 ccgatgcctt gcagcagggt gcctcgcagt ttgagcagca ggcgggcaag ctcaagagga
      841 aattctggct tcagaactta aagatgatga tcatcatggg cgtgattggc ctggttgtcg
      901 tgggcattat tgcaaataaa cttggactca taggcgggga gcagccgcca cagtaccagt
      961 atcccccaca gtacatgcag ccaccgccgc cgccaccgca gcagccagcc ggaggacagt
     1021 catcgctggt ggatgcggca ggagcaggag acggagcag
//
