LOCUS       sequence-138832-        1087 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 Est01.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 hydrolase/transferase enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK365
FEATURES             Location/Qualifiers
     source          1..1087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             482..504
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(535..554)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(536..554)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacggagc tcgaattctt atttgtcaac agcgcatggt ttcccgtcat catccaacgc
      121 cacaaaaatg aattttccct gcgtgacttt ctgccggatg gcggtgcgcc gtattctgcg
      181 aattgcctca acctcaattg tcatggatgt acggccaacc ttgagaatac gggtataaac
      241 gttcaattca tcccccaccg ccatggggcg atgaaatacc agcccgtcta tcgcaaccgt
      301 ggcacatttc cgccctgccc tgtcttcagc cgttgtgctg gcagccagat ccatatgagc
      361 agcaacccat ccaccaaaga catctccggc cgcattggta tcccgtcgca tcgccacaac
      421 acggacagca aggttatcgt catgtaccca gtaactctgc tcgccaccat ccatatgtat
      481 atctccttct taaagttaaa caaaattatt tctagaggga aaccgttgtg gtctccctat
      541 agtgagtcgt attaatttcg cgggatcgag atctcgggca gcgttgggtc ctggccacgg
      601 gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact
      661 ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg
      721 tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa
      781 acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg
      841 ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt
      901 tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac
      961 cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc
     1021 attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa
     1081 accgccc
//
