LOCUS       sequence-138840-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Mycobacterium tuberculosis FdxB (C-terminal flavoprotein 
            reductase domain).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Kappock
  TITLE     Additional fatty acid desaturase and acetylenase constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK126
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgccggccc gctccagctc gccgcacgcg
      121 gtgctgcacc gcatcccggt cgcgtcggtg gatcccatca ccgccgatgc caccctggtg
      181 actttcgcgg tgccggaagc attgcgggac gcgttccgat tcgagccggg ccagcacgtg
      241 acggtgcgca ccgacctggg cggccaaggc atccggcgca actactcgat ctgcgccccg
      301 gccacccgcg cccagctgcg catcgccgtc aaacacattc ccggcggggc gttttcgacg
      361 ttcgtggcca acgaactgaa ggccggcgac gtgctcgagc tgatgacacc gaccggccgg
      421 ttcggcaccc cgctggatcc gttgcaccgc aagcactatg tgggcctggt ggccggcagc
      481 gggatcaccc cggtgctgtc catcctggcg accacgctgg agatcgagac cgaaagccga
      541 ttcacgctga tctacggcaa ccgcaccaag gaatcgacga tgtttcgggc cgagctggat
      601 cgtctggagt cgcgctatgc cgaccggctg gaaatcctgc acgtgctctc cagcgagccg
      661 ctgcacaccc cggagctgcg cgggcgcatc gaccgagaca aactcaccag gtggctgacg
      721 agtaccctgc ggccggccgg tgtggacgaa tggttcatct gcggcccgct cgccatggcc
      781 accgcggtgc gcgagaccct gatcgagcac ggcgtggact ccgagcgcat tcacctggag
      841 ttgttctacg ggttcgacac gcccccggcg acccgtccct cctatgcggg agccaccgtc
      901 accttcacgc tgtccgggca gcgggcgata ttcgatctgg tgcccggcga ctcgattctg
      961 gaaggggcgc tggggctgcg ca
//
