LOCUS       sequence-138843-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase 
            (H6UctB) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Mullins EA, Starks CM, Francois JA, Sael L, Kihara D, Kappock TJ
  TITLE     Formyl-coenzyme A (CoA):oxalate CoA-transferase from the acidophile 
            Acetobacter aceti has a distinctive electrostatic surface and 
            inherent acid stability.
  JOURNAL   Protein Sci. 2012 May;21(5):686-96. doi: 10.1002/pro.2054. Epub 2012
            Mar 29.
  PUBMED    22374910
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2054"; 
            journalName: "Protein Sci"; date: "2012-05"; volume: "21"; issue: 
            "5"; pages: "686-96"
COMMENT     Sequence Label: pJK287
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgggcacga catcagaaaa ttccaaacct
      121 cttgatggta ttaaagtcat tgattttgga ggggtgcagt ctgttccttc agcggcgcag
      181 ctgttggcat ggtacggtgc tgatgtgatc aagattgagc gtgttggggt gggcgatatt
      241 acgcgtaacc agctgcggga tattccggat gcagatgcgc tatattttac aatgctcaat
      301 tgtaacaagc gttccgttga actgaacacc aagacaccgg aaggcaaggc tgtatttgaa
      361 aaatgcatca aatgggctga tattcttctt gaaaattttc gccccggtgc aatggagcgc
      421 atgggtttta cctgggaata tttgcagcag cttaacccgc gcctgattta cggcaccgtt
      481 aaggggtttg gtgaaaactc cccctgggct ggtgtgagcg cttatgaaaa cgttgcgcaa
      541 tgcgcgggtg gggcaacatc cacaacgggt tactggaatg gcgcgccact ggtggatggt
      601 caggcccccg gcaacaataa cgggccactg gtgagtgccg cagcacttgg tgatagcaat
      661 accggcaacc atttgttgat tggcgttctg gcagcactgt ttgggcgcga acggaccggc
      721 aaggggcaga aaatcagtgt ttccatgcag gatgccgtgc tcaatctctg tcgcgtcaag
      781 ctgcgtgatc agcaaagact ggaacgtgta gggtatctgg aagaatatcc gcagtatccg
      841 aacgggaagt ttggcgatac ggtgccgcgt ggcggcaatg ctggcggtgg tggtcagccc
      901 ggctggattt tgaagtgcaa aggctgggaa accgacgata acgcctatat ttactgcacg
      961 gtgcaggagc aggactgggg cccgacctgc gaagccattg gtaagccgga atgggcaaca
     1021 ga
//
