LOCUS       sequence-138846-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 succinyl-CoA:acetate 
            CoA-transferase, N-terminal His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 hydrolase/transferase enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK361
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgacagagc gcattcggaa cgtcgcgctg
      121 cgcagtaaag tatgtccggc agaaaccgca tccgagttga tcaagcacgg agacgtggtg
      181 gggaccagcg gttttaccgg ggcaggttac cccaaggaag tacccaaggc gctggcccag
      241 cggatggaag ctgcgcatga tcggggcgaa aaataccaga tcagcctgat tacgggcgca
      301 tccacgggcc ctcagctgga tggtgagctg gccaaggcca acggggtgta tttccgctcg
      361 ccattcaata cggatgccac catgcgcaac cgtattaatg cgggtgaaac ggaatatttt
      421 gataaccatc tggggcaggt ggctggccgt gccgtgcagg gcaattacgg caagttcaat
      481 attgcgctgg tggaagccac agccattacg gaagatggcg gcattgtgcc cacatcttct
      541 gtcggcaatt cccagacatt cctcaatctg gccgaaaagg tcattatcga ggtaaatgaa
      601 tggcagaatc cgatgctgga aggcatccat gatatctggg atggcaacgt aagtggcgtg
      661 cctacgcgtg atattgtgcc gattgtacgg gcagatcagc gcgtgggtgg cccggtttta
      721 cgggtgaacc cggacaagat tgctgccatt gtacgcacga atgaccggga cagaaatgcc
      781 ccgtttgccg cgccggatga aacagcaaaa gctattgcgg gctatttgct tgatttcttt
      841 gggcatgaag tcaaacagaa ccgtttgccg ccctcacttc tgccgctcca gtctggtgtg
      901 ggcaatgtgg ccaacgccgt gctggaaggg ctgaaggaag gcccatttga aaatctggtg
      961 ggctatagtg aggtgattca ggatggcatg ttggccatgc tggattctgg ccgtatgcgt
     1021 attgcctctg cctcgtcttt
//
