LOCUS       sequence-138854-        1106 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Neuronal-synaptobrevin (syb):spYFP1–10 chimera for synaptic GRASP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Macpherson LJ, Zaharieva EE, Kearney PJ, Alpert MH, Lin TY, Turan Z,
            Lee CH, Gallio M
  TITLE     Dynamic labelling of neural connections in multiple colours by 
            trans-synaptic fluorescence complementation.
  JOURNAL   Nat Commun. 2015 Dec 4;6:10024. doi: 10.1038/ncomms10024.
  PUBMED    26635273
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-12-4"; volume: "6:10024. doi"; pages: " 
            10.1038/ncomms10024"
COMMENT     Sequence Label: pLOT-syb-sp-YFP1-10
FEATURES             Location/Qualifiers
     source          1..1106
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(117..758)
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQTVLSKDPNEK"
ORIGIN
        1 tacacttaaa aactttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
       61 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc ctctagacta tgttcctttt
      121 tcatttggat ctttgctcag gactgtttgg tatgagaggt agtgattatc aggaagaagt
      181 accggaccgt ctccaatagg ggtgttttgt tggtaatggt cggcaagttg aacactgcca
      241 tcttctacat tatggcgtac tgtgaaattc gctttaattc cgttcttttg cttatcagca
      301 gtgatgtata cgttatgact attaaaattg tattcgagtt tgtgtccaag aatattacca
      361 tcttctttaa aatctgttcc tttcagttca atgcggttaa ccaaagtatc tccttcaaat
      421 ttaacaactg ctctagtttt atattttcca tcatctttga acgaaattgt tctctcttgc
      481 acgtaacctt caggcatagc cgacttaaaa aagtcatgtc ttttcatatg gtctgggtat
      541 ctcgcaaagc actgaagtcc gtacccaagc gttgttacga gagtcggcca tggtacagga
      601 agtttaccag tagtacagat gaatttaaga gtcaattttc caatggtggc gtcaccttct
      661 ccttcgcctc tcactgagaa cttatgtccg ttgacatcac catcgagttc aaccaaaatt
      721 ggcacaacac cggtaaacag ttcttctcct ttggacattc ctcgagccac gccgccgtga
      781 tcgccagctc ctgccgatcc tcctgctcct gctccgtctc ctgctcctgc cgcatccacc
      841 agcgatgact gtcctccggc tggctgctgc ggtggcggcg gcggtggctg catgtactgt
      901 gggggatact ggtactgtgg cggctgctcc ccgcctatga gtccaagttt atttgcaata
      961 atgcccacga caaccaggcc aatcacgccc atgatgatca tcatctttaa gttctgaagc
     1021 cagaatttcc tcttgagctt gcccgcctgc tgctcaaact gcgaggcacc ctgctgcaag
     1081 gcatcggcac ggtcgtccag ctccga
//
