LOCUS       sequence-138855-        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 putative SixA with C-terminal His6 
            tag (SixAH6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Mullins E, Kappock TJ
  TITLE     Functional analysis of the acetic acid resistance (aar) gene cluster
            in Acetobacter aceti strain 1023
  JOURNAL   Acetic Acid Bacteria. 2(s1):e3 (2013)
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acetic Acid
            Bacteria. 2(s1):e3 (2013)"
COMMENT     Sequence Label: pJK502
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             563..580
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(633..651)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      647..694
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(818..837)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgcgccgc cttgttctgc ttcgccacgc
       61 tgaagccgct tccgcccctt tgggaaattt cagcgttgat gctgacatga atcgcccgct
      121 tacgcccgca gggcaagagg cggcacagcg ctgtgggcac tggctggcag cgcacggcat
      181 tgtgcccgat accattttat gtagcccggc agtgcgcaca cggcagactc tggcaggcgt
      241 tgtggcggca ctggcatgcc cccttcccac gcccgaatat tgtgcagata tttacgacgc
      301 cacgcctgaa gctttgttgg cgcaaattca gcgcgttccc gattcagccc acacagtgct
      361 gatggtgggg cataaccccg gtatttcctt gctggcgcat cagttagaca tgcaggccag
      421 tgcgctggat cagggattcg ctccagcctc tgttgctgtt tttgaaatgt acggtagcga
      481 aacgttgcca gatataacac actggcaaat gtgcgatgcc caaagcatgc atttgcacac
      541 attcgcgcga ccctcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa
      601 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
      661 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt
      721 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      781 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
      841 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
      901 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
      961 cgtagtgggc catcgccctg atagacggtt tttcgccctt tga
//
