LOCUS       sequence-138856-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 putative SixA with C-terminal His6 
            tag (SixAH6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Mullins E, Kappock TJ
  TITLE     Functional analysis of the acetic acid resistance (aar) gene cluster
            in Acetobacter aceti strain 1023
  JOURNAL   Acetic Acid Bacteria. 2(s1):e3 (2013)
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acetic Acid
            Bacteria. 2(s1):e3 (2013)"
COMMENT     Sequence Label: pJK502
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             577..599
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(630..649)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(631..649)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgaggg tcgcgcgaat
       61 gtgtgcaaat gcatgctttg ggcatcgcac atttgccagt gtgttatatc tggcaacgtt
      121 tcgctaccgt acatttcaaa aacagcaaca gaggctggag cgaatccctg atccagcgca
      181 ctggcctgca tgtctaactg atgcgccagc aaggaaatac cggggttatg ccccaccatc
      241 agcactgtgt gggctgaatc gggaacgcgc tgaatttgcg ccaacaaagc ttcaggcgtg
      301 gcgtcgtaaa tatctgcaca atattcgggc gtgggaaggg ggcatgccag tgccgccaca
      361 acgcctgcca gagtctgccg tgtgcgcact gccgggctac ataaaatggt atcgggcaca
      421 atgccgtgcg ctgccagcca gtgcccacag cgctgtgccg cctcttgccc tgcgggcgta
      481 agcgggcgat tcatgtcagc atcaacgctg aaatttccca aaggggcgga agcggcttca
      541 gcgtggcgaa gcagaacaag gcggcgcata tgtatatctc cttcttaaag ttaaacaaaa
      601 ttatttctag agggaaaccg ttgtggtctc cctatagtga gtcgtattaa tttcgcggga
      661 tcgagatctc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt
      721 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcac
//
