LOCUS       sequence-138861-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase
            (AarC) with C-terminal His6 tag and Cys357>Tyr mutant 
            (AarCH6-C357Y).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Mullins E, Kappock TJ
  TITLE     Functional analysis of the acetic acid resistance (aar) gene cluster
            in Acetobacter aceti strain 1023
  JOURNAL   Acetic Acid Bacteria. 2(s1):e3 (2013)
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acetic Acid
            Bacteria. 2(s1):e3 (2013)"
COMMENT     Sequence Label: pJK504
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgatgat
       61 gggagcatgg tgcccgtatc aataaaccgc tgatgccaag acagagcttc cgtcagcaga
      121 tgcggtgtgt gcttaccaaa ggaattttta agcgcacgat caaaataatc ctgcaacatc
      181 ggccggtaat ccggatgcgc acatttggaa ataatttcac gcgcacgttg caccggtgaa
      241 agcccacgca gatccgccag cccctgctcg gttacaaaaa tctgcgcatc ctgcatgatg
      301 tggtccacat gcgcagccat cggcacaatg gctgaaatct tgccaccttt ggctgtggag
      361 ggagacagga agatggacag gtaggaactc cgggcaaaat cacccgatcc cccaatacca
      421 ttcatcattt tggaacccat cacgcgggta gaattcacgt ttccgtaaat atccgcctca
      481 atcatgccgt tcatggcaat gtagcctaaa cggcggataa tgccggggct gttgctgaca
      541 tcctgctggc gcagaatgat cttgctgcgg aagaaatcca tccggttatt gatttcttcc
      601 gcagcttccg ggctcagcga gaaagacgag gcagaggcaa tacgcatacg gccagaatcc
      661 agcatggcca acatgccatc ctgaatcacc tcactatagc ccaccagatt ttcaaatggg
      721 ccttccttca gcccttccag cacggcgttg gccacattgc ccacaccaga ctggagcggc
      781 agaagtgagg gcggcaaacg gttctgtttg acttcatgcc caaagaaatc aagcaaatag
      841 cccgcaatag ctttt
//
