LOCUS       sequence-138863-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2 vector with fli1ep element upstream of 6xmyc, attR1/R2 
            cassette, and late SV40 pA signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: 371 pTol2-fli1ep:MTDest
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(79..85,86..103,104..109))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(134..258)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(286..315)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(349..378)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(388..417)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(427..456)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(466..495)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(505..534)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgacggat
       61 cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag cctggggtgc
      121 ctaatgcggc cgccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt
      181 tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc
      241 ttttttgtac aaacttgtga tccttgaatt cgagatctct aaattcaagt cctcttcaga
      301 aatgagcttt tgctccatgg tgaggtcgcc caagctctcc atttcattca agtcctcttc
      361 agaaatgagc ttttgctcca tttcattcaa gtcctcttca gaaatgagct tttgctccat
      421 ttcattcaag tcctcttcag aaatgagctt ttgctccatt tcattcaagt cctcttcaga
      481 aatgagcttt tgctccattt cattcaagtc ctcttcagaa atgagctttt gctccatagc
      541 tttaaatcga tgctagcgat aattcgccct tcgcgtctga attaattcca gcccccgata
      601 tgtggcggag agacagagac agtttcgccg gatggttttt tttcctctaa atttgggaag
      661 tgaagtcact gcgcttgaga aggaagccgt gtatgcgtgt atgtgtgtgt gtgtatgtgt
      721 gtgtgtcact g
//
