LOCUS       sequence-138866-        1109 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express an NT-3-BDNF hybrid neurotrophic factor, with reduced 
            affinity to p75NTR, in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Enomoto M, Bunge MB, Tsoulfas P
  TITLE     A multifunctional neurotrophin with reduced affinity to p75NTR 
            enhances transplanted Schwann cell survival and axon growth after 
            spinal cord injury.
  JOURNAL   Exp Neurol. 2013 Oct;248:170-82. doi: 
            10.1016/j.expneurol.2013.06.013. Epub 2013 Jun 20.
  PUBMED    23792206
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Neurol."; date: "2013-10"; volume: "248:170-82. doi"; pages: " 
            10.1016/j.expneurol.2013.06.013. Epub 2013 Jun 20"
COMMENT     Sequence Label: pLV-75-2
FEATURES             Location/Qualifiers
     source          1..1109
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1036..1060)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(1057..1108)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(1086..1106)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aaggagcaac atagttaaga ataccagtca atctttcaca aattttgtaa tccagaggtt
       61 gattgtcgac ggtatcgata agcttgataa cttataatat ataaaaacaa tataaacatt
      121 tatatgctac atgcatatca tataatttaa agtaataatt tatatatggg gagagatgcc
      181 aattcatgtt cttccgattg ctgcagacaa ggcacacaca caggacgtgt ctatccgtat
      241 ccaccgccag cccacgagtt tattgttctc tgaagtcagt gctcggacgt aggtttggga
      301 tgttttgcac tgagagttcc agtgtttatc atcaataccc ctgcaaccgt ttttgaccgg
      361 cctggcttcc ttacatcgcg tttcataaaa atattgtttg acgggagagt tgcccgtttt
      421 gatctccccc agcaccgtga cctggtgtcc ccgaatgtcg atggccgatg acttgtcggt
      481 cacccacaag ctttcactgg cacataccga gtactcccct cggtgactct tatgctccgc
      541 gtaccgtttc cgccgtgatg ttctgttcgc caccacgggg ctgcccacgt aatcctccat
      601 gagatacaag ggcgggggct ccaagggggt gctgtcgctc agcaggaccc gcggtgagtt
      661 gtagcgtctc tgttgtcgca gcagttcggt gtccattgca atcaccggct ggaatgctga
      721 cttggcgggc cctccccgct ccggctctcg gggagcctca gctttgggca gggtgctctg
      781 gtaattttcc ttaacgtcca ccatctgctt ggagagcttg tttttcaaaa tatctgcctg
      841 gatcagctta ataatgaggg aattgagcga gtcttctggc aaactccttt gatccatgtt
      901 gttaccttgg atgccacgga gataagcgag aaatatcaca taaaacaaga tggacatggt
      961 cttgttcacc tgtaggatcc tctagagtcg gtgtcttcta tggaggtcaa aacagcgtgg
     1021 atggcgtctc caggcgatct gacggttcac taaacgagct ctgcttatat agacctccca
     1081 ccgtacacgc ctaccgccca tttgcgtca
//
