LOCUS       sequence-138870-        7179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Crepis alpina delta-8 sphingolipid desaturase (SLD1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Kappock
  TITLE     Additional fatty acid desaturase and acetylenase constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK275
FEATURES             Location/Qualifiers
     source          1..7179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(66..83)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(237..825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(317..336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(996..1787,1788..1856))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCDTLLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLID*MEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(1952..2755)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(2756..2971)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     rep_origin      3572..4452
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     rep_origin      complement(4521..4976)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4870..4889
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4995..5437
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    4995..5112
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     5408..5431
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     5469..5488
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5469..5487
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      6928..7175
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6944..6962)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 tgagcaaaag gccagcaaaa gcccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      421 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      661 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1081 ataactacga tacgggagcg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1201 agaagtggtc ctgcaacttt atccgcctcc attcagtcta ttaattgttg ccgggaagct
     1261 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttggcattgc tacaggcatc
     1321 gtggtgtcac tctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1381 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1441 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1501 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1561 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1621 aatagtgtat cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1681 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1741 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1801 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1861 ttcctttttc aatgggtaat aactgatata attaaattga agctctaatt tgtgagttta
     1921 gtatacatgc atttacttat aatacagttt tttagttttg ctggccgcat cttctcaaat
     1981 atgcttccca gcctgctttt ctgtaacgtt caccctctac cttagcatcc cttccctttg
     2041 caaatagtcc tcttccaaca ataataatgt cagatcctgt agagaccaca tcatccacgg
     2101 ttctatactg ttgacccaat gcgtctccct tgtcatctaa acccacaccg ggtgtcataa
     2161 tcaaccaatc gtaaccttca tctcttccac ccatgtctct ttgagcaata aagccgataa
     2221 caaaatcttt gtcgctcttc gcaatgtcaa cagtaccctt agtatattct ccagtagata
     2281 gggagccctt gcatgacaat tctgctaaca tcaaaaggcc tctaggttcc tttgttactt
     2341 cttctgccgc ctgcttcaaa ccgctaacaa tacctgggcc caccacaccg tgtgcattcg
     2401 taatgtctgc ccattctgct attctgtata cacccgcaga gtactgcaat ttgactgtat
     2461 taccaatgtc agcaaatttt ctgtcttcga agagtaaaaa attgtacttg gcggataatg
     2521 cctttagcgg cttaactgtg ccctccatgg aaaaatcagt caagatatcc acatgtgttt
     2581 ttagtaaaca aattttggga cctaatgctt caactaactc cagtaattcc ttggtggtac
     2641 gaacatccaa tgaagcacac aagtttgttt gcttttcgtg catgatatta aatagcttgg
     2701 cagcaacagg actaggatga gtagcagcac gttccttata tgtagctttc gacatgattt
     2761 atcttcgttt cctgcaggtt tttgttctgt gcagttgggt taagaatact gggcaatttc
     2821 atgtttcttc aacactacat atgcgtatat ataccaatct aagtctgtgc tccttccttc
     2881 gttcttcctt ctgttcggag attaccgaat caaaaaaatt tcaaggaaac cgaaatcaaa
     2941 aaaaagaata aaaaaaaaat gatgaattga aaagctagct tatcgatgat aagctgtcaa
     3001 acatgagaat taattccacg gactatagac tatacctagt atactccgtc tactgtacga
     3061 tacacttccg ctcaggtcct tgtcctttaa cgaggcctta ccactctttt gttactctat
     3121 tgatccagct cagcaaaggc agtgtgatct aagattctat cttcgcgatg tagtaaaact
     3181 agctagaccg agaaagagac tagaaatgca aaaggcactt ctacaatggc tgccatcatt
     3241 attatccgat gtgacgctgc agcttctcaa tgatattcga atacgctttg aggagataca
     3301 gcctaatatc cgacaaactg ttttacagat ttacgatcgt acttgttacc catcattgaa
     3361 ttttgaacat ccgaacctgg gagttttccc tgaaacagat agtatatttg aacctgtata
     3421 ataatatata gtctagcgct ttacggaaga caatgtatgt atttcggttc ctggagaaac
     3481 tattgcatct attgcatagg taatcttgca cgtcgcatcc ccggttcatt ttctgcgttt
     3541 ccatcttgca cttcaatagc atatctttgt taacgaagca tctgtgcttc attttgtaga
     3601 acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac
     3661 agaacagaaa tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt
     3721 gtaaaacaaa aatgcaacgc gacgagagcg ctaatttttc aaacaaagaa tctgagctgc
     3781 atttttacag aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact
     3841 tcttttttgt tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag
     3901 attacttttt ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg
     3961 taggtccgtt aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa
     4021 gcctgactcc acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa
     4081 gataaaggca tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag
     4141 aaagtgatag cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt
     4201 tgtctctata tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct
     4261 atgaatagtt cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa
     4321 aatgtagagg tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata
     4381 tagggatata gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc
     4441 ggtattcgca atgggaagct ccaccccggt tgataatcag aaaagcccca aaaacaggaa
     4501 gattgtataa gcaaatattt aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa
     4561 tttttgttaa atcagctcat tttttaacga atagcccgaa atcggcaaaa tcccttataa
     4621 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttccaaca agagtccact
     4681 attaaagaac gtggactcca acgtcaaagg gcgaaaaagg gtctatcagg gcgatggccc
     4741 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcagtaaa
     4801 tcggaagggt aaacggatgc ccccatttag agcttgacgg ggaaagccgg cgaacgtggc
     4861 gagaaaggaa gggaagaaag cgaaaggagc gggggctagg gcggtgggaa gtgtaggggt
     4921 cacgctgggc gtaaccacca cacccgccgc gcttaatggg gcgctacagg gcgcgtgggg
     4981 atgatccact agtacggatt agaagccgcc gagcgggtga cagccctccg aaggaagact
     5041 ctcctccgtg cgtcctcgtc ctcaccggtc gcgttcctga aacgcagatg tgcctcgcgc
     5101 cgcactgctc cgaacaataa agattctaca atactagctt ttatggttat gaagaggaaa
     5161 aattggcagt aacctggccc cacaaacctt caaatgaacg aatcaaatta acaaccatag
     5221 gatgataatg cgattagttt tttagcctta tttctggggt aattaatcag cgaagcgatg
     5281 atttttgatc tattaacaga tatataaatg caaaaactgc attaaccact ttaactaata
     5341 ctttcaacat tttcggtttg tattacttct tattcaaatg taataaaagt atcaacaaaa
     5401 aattgttaat atacctctat actttaacgt caaggagaaa aaaccccgga tcggactact
     5461 agcagctgta atacgactca ctatagggaa tattaagctt ggtaccgagc tcggatccac
     5521 gatggtttct ccttccgttg ctgtcgttga agtattgaag tcaacaagca gcagcgacgg
     5581 aaagaaatac ataacatcag aagagctaga aaaacacaac aaaccgacgg acctatggat
     5641 ctcaatacaa ggcaaggtgt acaacgtgac ggaatggtcg aaaatccacc cgggggggga
     5701 catccccctc ctgaacctgg cgggccagga cgtgacggac gccttcatcg ccttccaccc
     5761 ggggtccgcc tggcaccacc tccaaagcct cttcacgggc taccacctcc aagactacca
     5821 agtctcggac atctctaaag actaccgcaa gctcgcgtcc gagttttcaa aagccggaat
     5881 gttcgacaag aaagggcacg gtgtcatcta ctccctctgc ttcgtctcca tcctcctttc
     5941 cctttgcgtg tacggcgtcc tgtgctccgc tagtgtcacc gtccacatgt ttgcgggcgc
     6001 gctgctgggc ttggcgtgga tgcaaatcgc gtatctgggc cacgacgcgg gtcattacca
     6061 gatgatggcg acccgcgggt ggaacaagtt tgccgggatt ttcatcggga actgtatcac
     6121 gggtattagc atcgcgtggt ggaagtggac ccacaatgcc caccacatcg cgtgcaactc
     6181 cctcgattac gaccccgact tgcaacacct ccccatgctc gcggtttcct cgaagctatt
     6241 cgagtccatc acttcggttt tttacgggag aagattaacg ttcgatagcg tcgcgagatt
     6301 cttcatctcg taccaacact acacttacta ccccatcatg tgtgtgacac gtgtgaacct
     6361 ctacctacaa acaatcctac tactatgctc aaaacgaaga atccctgacc ggaccttaaa
     6421 catcatcggg acgcttatct tttggacttg gtttcctctt ttagtgtcct gtctccccaa
     6481 ctggcctgaa cgtgtcgcct ttgtgctggt ttcgttttgt gtgaccggga tccaacatgt
     6541 gcagtttaca ctaaaccatt tcgcggctga cgtgtacgtg ggcccgccga gagggaacga
     6601 ttggtttgag aagcaaacgg ccgggaccat cgacatttcg tgttcttcct acatggattg
     6661 gttttttggc gggttgcagt tccaactcga gcatcatttg tttccgaggt tacctaggtg
     6721 tcacttgagg tcgatttctc cgattgtgat ggatctttgt aagaagcata acttgccgta
     6781 tacgagtttg tctttctttg atgcgaatgt gacgactctt aagacactta ggactgcggc
     6841 gttgcaggcg cgtgacctgg cgaatggccc cccacagaac ttagtttggg aagcgtttaa
     6901 tactcatggt tgatctagag ggccgcatca tgtaattagt tatgtcacgc ttacattcac
     6961 gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg
     7021 tccctattta tttttttata gttatgttag tattaagaac gttatttata tttcaaattt
     7081 ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg
     7141 agaaggtttt gggacgctcg aaggctttaa tttgcggcc
//
