LOCUS       sequence-138884-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with 
            c-myc tag and His6-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Kappock
  TITLE     Additional fatty acid desaturase and acetylenase constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK137
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        74..1012
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
ORIGIN
        1 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
       61 ggtcatgaga tcagatctaa catccaaaga cgaaaggttg aatgaaacct ttttgccatc
      121 cgacatccac aggtccattc tcacacataa gtgccaaacg caacaggagg ggatacacta
      181 gcagcagacc gttgcaaacg caggacctcc actcctcttc tcctcaacac ccacttttgc
      241 catcgaaaaa ccagcccagt tattgggctt gattggagct cgctcattcc aattccttct
      301 attaggctac taacaccatg actttattag cctgtctatc ctggcccccc tggcgaggtt
      361 catgtttgtt tatttccgaa tgcaacaagc tccgcattac acccgaacat cactccagat
      421 gagggctttc tgagtgtggg gtcaaatagt ttcatgttcc ccaaatggcc caaaactgac
      481 agtttaaacg ctgtcttgga acctaatatg acaaaagcgt gatctcatcc aagatgaact
      541 aagtttggtt cgttgaaatg ctaacggcca gttggtcaaa aagaaacttc caaaagtcgg
      601 cataccgttt gtcttgtttg gtattgattg acgaatgctc aaaaataatc tcattaatgc
      661 ttagcgcagt ctctctatcg cttctgaacc ccggtgcacc tgtgccgaaa cgcaaatggg
      721 gaaacacccg ctttttggat gattatgcat tgtctccaca ttgtatgctt ccaagattct
      781 ggtgggaata ctgctgatag cctaacgttc atgatcaaaa tttaactgtt ctaaccccta
      841 cttgacagca atatataaac agaaggaagc tgccctgtct taaacctttt tttttatcat
      901 cattattagc ttactttcat aattgcgact ggttccaatt gacaagcttt tgattttaac
      961 gacttttaac gacaacttga gaagatcaaa aaacaactaa ttattcgaaa cgaggaattc
     1021 acgtggccca gccggc
//
