LOCUS       sequence-138898-         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Production of Acetobacter aceti 1023 alanine racemase (alr) with 
            N-terminal His tag and Ala367>Asp mutant (H6AaAlr-A367D).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Francois JA, Kappock TJ
  TITLE     Alanine racemase from the acidophile Acetobacter aceti.
  JOURNAL   Protein Expr Purif. 2007 Jan;51(1):39-48. Epub 2006 Jun 6.
  PUBMED    16843006
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2007-01"; volume: "51(1)"; pages: "39-48. Epub 
            2006 Jun 6"
COMMENT     Sequence Label: pJK325
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt
       61 caggcatccg ttgcataaac gcgatgataa cgccgcccca gagatgtcag tacttcgtag
      121 cctattgtgc cttctgcttt ggccacagca tctacgccgt aagactcatt caaaagttcc
      181 accatgctat cttcttccag cagcccttcg ggcactgcgg aaagatcaac tgtcatggaa
      241 tccatagaga ttcggcccag aattggtagc cgatatcctt tataccaagc gcatcctgca
      301 gcagcgcccg cacgggcaaa tccatcagca tatccaaccg caatggtcgc aacacggcat
      361 gggccagtcg cttgccacgt aagcccatat cccacccgat cccctttgga taaagtgcga
      421 atttgcagaa tatgcgtttg caaccccaca acaggcttaa gtggattaac accatcattc
      481 tgcggcgcta cgccatacaa agcggcacca gggcgcacga gatcaaaatg aaatggttgc
      541 cccagaaaaa ttccagaaga tgcagaaaga cttaaagctg cctttggcaa catgcgtgca
      601 tagttctcaa accgttcacg ctgcatggca ttggctgcgt tttctggcgt atctgcacag
      661 gccagatgac tcataaccag actgacagat aacccctcca aaagttcagg agtggccatc
      721 agtttatgga tatcagcctc tgaaaggcca aaacgagaca tacccgtatc cagttgaatg
      781 gccacatcca gcggcttatc cattttggct gccagatccc gcaaaacagc cacctgtttt
      841 aggctattta aaactggccg caaattattg cgtacgcaaa cctcagcagc atccggccgt
      901 ggaccatgca acacagttac tgatgtgtgg ggtgaaagat atggcctaag ggctagccct
      961 tcatccacat gtgcgacaaa aaaat
//
