LOCUS       sequence-138925-        7364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Sema3C protein (truncated at cleavage site P1; ie, 
            short), C-terminally fused to the Fc region of human IgG1 + 6X 
            histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 7364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema3c(S)-Fc-His
FEATURES             Location/Qualifiers
     source          1..7364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3944..3961
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3987..4108
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4024..4043)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4078..4097
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4146..4163)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4317..4905)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4397..4416)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5076..5867,5868..5936))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5699..5718
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5937..6041)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6109..6127
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6165..6187)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6189..6209)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6194..6524
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6375..6510
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6437..6456
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6661..6680
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6747..7202)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6884..6905)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7096..7115
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7328..7350
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7342..7359
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7343..7359
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggcattccg
     1621 ggcgatttgt gtgttggttg gagtatttat ttgttccatt tgtgtacgag gatcttccca
     1681 gccccaagca agagtttatt taacatttga tgagcttcga gaaaccaaaa cctctgagta
     1741 ctttagtctg tcccaccagc agttagacta cagaatattg ctgatggatg aagatcaaga
     1801 ccggatatat gtggggagca aagaccacat cctgtctttg aatatcaaca atatcagtca
     1861 agaacctttg agtgttttct ggccagcatc aacaatcaaa gttgaagagt gcaaaatggc
     1921 tggcaaagat cctacacatg gctgtggaaa tttcgtccgg gtgattcaga cattcaaccg
     1981 tactcacctg tatgtctgtg ggagtggagc gttcagccca gtgtgcacct acctgaaccg
     2041 gggaaggagg tcagaggacc aggtattcat gatcgactct aagtgtgaat ctggcaaagg
     2101 acgatgctct ttcaacccga atgtgaacac tgtgtctgtt atgatcaatg aggaactctt
     2161 ctcaggaatg tatatagact tcatgggaac agatgctgct attttccgaa gtttaactaa
     2221 gaggaatgca gttcgaactg atcaacataa ttcaaaatgg ctgagtgaac ccatgtttgt
     2281 ggacgcacat gtgatcccag atggcactga tccaaatgat gctaaggtct atttcttctt
     2341 caaagaaaga ctgactgaca acaataggag cacaaaacag attcattcca tgattgcaag
     2401 aatatgccct aatgacactg gtggacaacg tagtcttgtc aacaagtgga ccacattctt
     2461 aaaggcaaga cttgtatgct cagtcacaga tgaagatggc ccagagacac attttgatga
     2521 actagaggat gtctttctgc tggaaactga caatccaagg acaacactcg tgtatggcat
     2581 cttcaccaca tcaagctctg tttttaaggg atcggcagtg tgtgtgtatc atttatctga
     2641 tatacagact gtattcaatg ggccctttgc ccacaaggaa gggcccaatc accagctgat
     2701 ctcctatcaa ggtagaatcc catatcctcg cccaggaact tgcccaggag gggcctttac
     2761 acccaatatg agaaccacca aggacttccc agatgacgtt gtcactttta ttcggaacca
     2821 ccctctcatg tacaattcca tctaccccat ccacagaagg cctctgatag tccgcatagg
     2881 cactgactac aagtacacaa agattgctgt ggaccgtgtc aacgctgctg atgggagata
     2941 ccacgttctg tttctgggca cagatcgggg caccgtgcag aaggtcgtag tccttcctac
     3001 caatagctct gccagtgggg aactcatcct ggaggagctg gaagtcttca agaatcatgt
     3061 tcccataaca acaatgaaaa tctcatccaa aaagcaacag ttgtacgtga gctccaatga
     3121 gggggtttcc caagtctctc tgcatcgctg ccatatctac ggcacagcct gtgcggactg
     3181 ctgcttggcg agggatccat actgtgcctg ggatggccac tcttgctcta ggttctaccc
     3241 cactgggggc gcgcctgaca aaactcacac atgcccaccg tgcccagcac ctgaactcct
     3301 ggggggaccg tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg
     3361 gacccctgag gtcacatgcg tggtggtgga cgtgagccac gaagaccctg aggtcaagtt
     3421 caactggtac gtggacggcg tggaggtgca taatgccaag acaaagccgc gggaggagca
     3481 gtacaacagc acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa
     3541 tggcaaggag tacaagtgca aggtctccaa caaagccctc ccagccccca tcgagaaaac
     3601 catctccaaa gccaaagggc agccccgaga accacaggtg tataccctgc ccccatcccg
     3661 ggatgagctg accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctatcccag
     3721 cgacatcgcc gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacgcc
     3781 tcccgtgctg gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag
     3841 caggtggcag caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca
     3901 ctacacgcag aagagcctct ccctgtctcc gggtaaaggc ggccatcatc accatcacca
     3961 ttaatctaga gatcttatta aagcagaact tgtttattgc agcttataat ggttacaaat
     4021 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4081 gtttgtccaa actcatcaat gtatcttatc atgtctggtc gactctagct agactcttcc
     4141 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4201 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     4261 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4321 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     4381 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4441 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4501 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4561 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4621 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     4681 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     4741 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     4801 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4861 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4921 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4981 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     5041 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     5101 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     5161 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     5221 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     5281 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     5341 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     5401 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     5461 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     5521 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     5581 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     5641 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     5701 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     5761 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     5821 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5881 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     5941 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6001 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6061 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     6121 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     6181 ctcccggagg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc caggcaggca
     6241 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     6301 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     6361 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6421 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     6481 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     6541 gtatatccat tttcgtcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     6601 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     6661 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     6721 gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc
     6781 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     6841 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     6901 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     6961 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     7021 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     7081 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     7141 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     7201 gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     7261 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     7321 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aatt
//
