LOCUS       sequence-138948-        1187 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Membrane scaffold protein (MSP), helix 4 deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Hagn F, Etzkorn M, Raschle T, Wagner G
  TITLE     Optimized phospholipid bilayer nanodiscs facilitate high-resolution 
            structure determination of membrane proteins.
  JOURNAL   J Am Chem Soc. 2013 Feb 6;135(5):1919-25. doi: 10.1021/ja310901f. 
            Epub 2013 Jan 25.
  PUBMED    23294159
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja310901f";
            journalName: "J Am Chem Soc"; date: "2013-02-6- 6"; volume: "135"; 
            issue: "5"; pages: "1919-25"
COMMENT     Sequence Label: pET28a-MSP1D1delltaH4
FEATURES             Location/Qualifiers
     source          1..1187
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..81
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             620..637
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(690..708)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      704..751
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      788..1168
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(875..894)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1085..1106
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcat
       61 gaaaacctgt attttcaggg cagcaccttt agcaaactgc gtgaacagct gggcccggtg
      121 acccaggaat tttgggataa cctggaaaaa gaaaccgaag gcctgcgtca ggaaatgagc
      181 aaagatctgg aagaggtgaa agcgaaagtg cagccgctgc gtgcggaact gcaggaaggc
      241 gcgcgtcaga aactgcatga actgcaggaa aaactgagcc cgctgggcga agagatgcgt
      301 gatcgtgcgc gtgcgcatgt ggatgcgctg cgtacccatc tggcgccgta tagcgatgaa
      361 ctgcgtcagc gtctggcggc ccgtctggaa gcgctgaaag aaaacggcgg tgcgcgtctg
      421 gcggaatatc atgcgaaagc gaccgaacat ctgagcaccc tgagcgaaaa agcgaaaccg
      481 gcgctggaag atctgcgtca gggcctgctg ccggtgctgg aaagctttaa agtgagcttt
      541 ctgagcgcgc tggaagagta taccaaaaaa ctgaacaccc agtaagagct ccgtcgacaa
      601 gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc
      661 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
      721 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc
      781 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
      841 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
      901 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
      961 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     1021 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     1081 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     1141 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatga
//
