LOCUS       sequence-138951-        9822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 9822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pFOSGE_DUM1H01
FEATURES             Location/Qualifiers
     source          1..9822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2416..2425
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(4367..4384)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgatggccg atcacttaga cgaaccgccc caaaagcggg ttaaaatgga tccaacggat
       61 atctcttact ttctggagga gaacctgccc gatgagctgg tgtcctcgaa tagtggctgg
      121 tcggatcagc tgaccggcgg agcaggcggt ggcaatggag gtggcggcgc ctccggtgta
      181 accacaaatc ccacatccgg cccaaatccc ggtggcggac ccaacaagcc ggcagcccaa
      241 ggacccggct ctggcacagg cggagtcggt gttggagtga atgtgggtgt cggcggtgtt
      301 gttggcgtcg gcgttgtgcc ttcccagatg aacggagccg gcggcggcaa cggatccgga
      361 acgggtggcg acgacggcag tggcaacggc tcaggagcgg gcaacagaat cagtcaaatg
      421 caacaccagc aactgcagca cctactccag cagcagcagg gccagaaggg cgccatggtg
      481 gtgcccggca tgcagcagct gggcagcaag tcgcccaacc tgcagtcacc caaccagggc
      541 ggcatgcagc aggtggtggg cactcagatg ggtatggtca actcaatgcc catgtcaata
      601 tcgaataatg gcaacaatgg catgaacgcc ataccaggca tgaacaccat tgcgcagggc
      661 aatctgggaa acatggtgct gaccaacagc gttggcggcg gcatgggcgg catggttaat
      721 catcttaagc agcagcctgg cggcggcggc ggtgggatga tcaattccgt ttcagtaccc
      781 ggaggacctg gagcaggagc tggtggcgtt ggagctggcg gcggaggagc cgttgccgca
      841 aaccaaggca tgcatatgca gaacggccca atgatgggac gcatggtggg gcaacagcat
      901 atgcttcgtg gcccgcatct catgggtgcc tctggaggag ctggtgggcc aggaaacggg
      961 cctggtggcg gaggaccacg catgcagaat ccgaacatgc aaatgactca actcaacagt
     1021 ctgccctacg gagtgggtca gtatggtggc ccaggcggtg gtaacaatcc tcagcaacag
     1081 cagcagcaac agcagcaaca acttctcgcc cagcagatgg cccaaagagg tggcgtcgta
     1141 ccgggcatgc cgcagggtaa tcggcccgtt ggcacagtgg tgcccatgtc cacactcggc
     1201 ggcgatggat cagggcccgc ggggcagctg gtaagcggga atcctcagca gcagcagatg
     1261 ctggcgcagc agcaaaccgg agccatgggc ccgcgtcctc cgcaaccaaa ccagctgctc
     1321 ggtcatcccg gccagcagca gcagcagcaa cagcagcctg gcacctcgca gcagcagcaa
     1381 cagcagcagg gagtcggaat cggaggagca ggcgttgtgg ccaatgcagg aaccgtggct
     1441 ggcgtgccgg cagtggcagg cggcggagcc ggtggtgccg tacaatctag cggccctggt
     1501 ggcgccaatc gcgatgtgcc cgacgaccgt aagcgacaga tccagcagca actgatgctg
     1561 ctcctccatg cacacaaatg caatcgcagg gagaacctga atccgaacag ggaagtgtgc
     1621 aacgttaact actgcaaggc gatgaaatcc gtgctggccc acatgggcac ttgcaaacag
     1681 agcaaggact gcaccatgca gcattgtgcc tcttcgcgcc aaattctgtt gcattataaa
     1741 acgtgccaga acagtggctg cgtcatttgc tatcccttcc ggcagaatca ttcggttttt
     1801 caaaatgcga atgtgccgcc aggaggcgga ccggcaggaa ttggaggtgc gccaccaggt
     1861 ggcggcggag cgggtggtgg agcggctgga gcaggcggta atcttcagca gcaacagcag
     1921 cagcaacaac agcagcagca gaaccagcag cccaatctga cgggtctggt agtggatggc
     1981 aagcaaggac agcaggttgc accgggaggt ggccaaaata ctgccatagt tcttccccag
     2041 caacagggag cgggcggtgc accgggtgcg ccgaaaacgc ctgcggatat ggtgcaacaa
     2101 ttgacccaac agcagcagca gcagcaacag caggttcacc agcaacaggt tcagcaacag
     2161 gaactccgtc gattcgatgg catgagccag caagtcgtag caggtggtat gcaacagcag
     2221 cagcagcagg gtttgcctcc tgtgattcgc attcaaggcg ctcagccggc cgtcagggta
     2281 ctgggaccag gtggtcccgg cggcccaagt ggaccaaatg ttctgccgaa cgatgttaac
     2341 agcctgcatc aacaacagca acaaatgctg caacagcagc agcaacaggg ccagaatcga
     2401 cgacgcggtg gcctggccac catggtggag caacaacagc agcatcagca acaacagcag
     2461 caacccaatc ccgcccagct gggtggcaac attccagcac cactctctgt caacgtcggt
     2521 ggctttggca ataccaattt cggtggtgca gctgccggcg gagccgtggg agccaacgat
     2581 aagcagcaac tgaaagtggc ccaagtgcat ccgcagagcc atggcgtagg agcgggcggt
     2641 gcatcagcgg gcgccggggc gagtggtggt caagtggcag ccggttccag tgtcctgatg
     2701 ccagccgata ccacgggcag tggtaatgcg ggcaatccca accagaatgc aggcggtgta
     2761 gctggaggtg ccggcggtgg caatggcgga aacactggac ctccgggcga caacgagaaa
     2821 gactggcggg aatcggtgac cgccgatctg cgcaaccacc tcgtccacaa actggtgcag
     2881 gccatcttcc ccacctcgga tcctacgacc atgcaggaca aacggatgca taatctcgtt
     2941 tcatacgcgg aaaaggtcga gaaggacatg tacgaaatgg ccaagtccag atcggagtac
     3001 tatcacctgc tggccgagaa gatctacaag attcaaaagg agctggagga gaagcgactg
     3061 aagcgtaagg agcagcatca gcagatgctg atgcagcaac agggcgttgc gaatccagtg
     3121 gctggaggag cggctggcgg agctggcagt gcagctggtg tagcgggagg tgtagtcttg
     3181 ccccagcagc aacagcagca gcaacaacaa cagcagcagc agggtcagca gcctctgcag
     3241 agctgtatcc atccaagcat cagtccaatg ggcggtgtga tgccgccgca gcagctgcgt
     3301 ccacagggac cacctggaat actgggccaa cagacggcag taggcctggg cgtcggcgtg
     3361 ggagtgacca acaatatggt aaccatgcgc agtcattcgc ccggtggcaa catgctcgcc
     3421 ttgcagcaac aacagcgcat gcagttcccg caacaacagc agcaacaacc gcccgggtct
     3481 ggagccggca aaatgctggt cggtccacca ggacccagtc ccggtggcat ggtggtcaat
     3541 cccgcgctct cgccttacca gacgaccaat gtgctcacca gtccagtgcc aggacagcag
     3601 caacagcagc agttcattaa tgcgaacggc ggcactggcg ccaatcctca actgagcgaa
     3661 atcatgaagc agcgtcacat tcaccagcag cagcaacaac aacagcagca gcagcagcag
     3721 ggaatgttgt tgccgcagtc gccatttagc aattcaacac ctctacaaca acaacagcag
     3781 cagcagcagc aacaacagca gcagcaggcg actagcaaca gttttagctc accaatgcag
     3841 caacagcagc aaggtcagca acagcaacaa cagaagcccg gcagtgtgct gaataatatg
     3901 ccgcccacgc ccacgagtct ggaagccctg aatgcggggg ccggagcgcc cggaactgga
     3961 ggatccgcct ccaatgtaac ggtttcagct ccgagcccat cgcctggctt cctgtccaac
     4021 ggcccgtcga ttggcacgcc ctccaacaat aataatagta gtagtgctaa caacaacccg
     4081 ccctcggtga gcagtctaat gcaacagccg ctgagcaatc ggccgggtac gcctccttac
     4141 atacccgctt ccccagtgtc ggcgacaagt gcctccggat tagcggcgag cagtacgccc
     4201 gcatcagcag cagccacctg tgcgagtagt ggcagtggca gcaatagcag cagcggagca
     4261 actgcagcgg gtgcaagttc cacgtcatca tcttcctcgg cgggctcggg tacaccactc
     4321 agctcggtat cgactcctac atcggccacg atggccacca gcagcggtgg tggtggtggt
     4381 ggtgggggca atgcaggagg cggatcatcc actacgcccg ctagcaatcc actgctcctc
     4441 atgtctggag gaacggcagg aggcggaacg ggagcaacga ccaccacatc gacatcctcg
     4501 agcagtcgca tgatgagcag ctccagcagt ctctcctcac agatggctgc cctggaggct
     4561 gcggcgcgag acaacgacga tgagacgccc tcgccatccg gcgagaatac gaacggcagt
     4621 ggtggcagtg gaaatgccgg cggtatggcc tccaagggca aactggactc cattaagcaa
     4681 gatgatgata tcaagaagga gtttatggat gacagctgtg gcggaaataa cgatagctcg
     4741 cagatggatt gctcgacggg tggtggcaag ggcaagaatg tgaacaacga cggaacaagc
     4801 atgatcaaaa tggagatcaa gacggaggat ggactcgatg gcgaggtaaa gatcaaaacg
     4861 gaggccatgg atgtggacga ggctggagga tcgacagccg gagagcatca tggcgaaggt
     4921 ggcggcggca gtggtgttgg cggcggtaag gataacataa atggtgcgca cgatggcgga
     4981 gcgacaggcg gtgctgtgga cataaaaccc aagacggaga cgaaaccact cgtaccggag
     5041 ccactggcac ccaatgcagg tgacaagaaa aagaagtgcc aattcaatcc cgaggaactg
     5101 cgcaccgctc tcctgccaac gctagagaag ctctacaggc aggagcccga atccgtgccc
     5161 tttcgctacc cagttgatcc ccaggcgctg ggcatacctg attactttga aatcgttaag
     5221 aagcccatgg acctgggcac tatacgcacc aacatccaga atggaaagta cagtgatccc
     5281 tgggaatatg tggacgacgt ttggctgatg ttcgacaatg cctggctgta taatcgcaaa
     5341 acatcgcggg tctatcgcta ttgcacaaag ctttccgaag tctttgaggc ggagattgat
     5401 cctgtgatgc aggcactggg atattgctgc ggcaggaagt acacattcaa tccacaggtg
     5461 ctatgctgct acggcaagca gctctgcacg attccgcggg atgccaagta ctacagctac
     5521 cagaacagtc taaaggaata cggtgtcgcc tcaaatagat acacctactg ccaaaagtgc
     5581 tttaacgaca tccagggcga tacggtcaca ctgggcgacg atccactgca atcgcaaacc
     5641 caaatcaaaa aggatcagtt caaggagatg aagaacgatc acctcgaact ggagccgttt
     5701 gtcaattgcc aggagtgcgg acgcaaacag caccaaatct gcgtactctg gctggattct
     5761 atctggcccg gtggcttcgt gtgcgataac tgcctgaaaa agaagaactc aaagcggaag
     5821 gagaacaagt tcaatgcgaa acgcctgccc accaccaagc tgggcgtgta catagagacg
     5881 cgggtgaata atttcctcaa gaagaaggag gctggtgccg gcgaggtgca cattcgtgtg
     5941 gtcagctcat cggacaagtg tgtagaggtg aagcccggca tgcgtcgacg attcgttgag
     6001 cagggcgaga tgatgaacga gttcccatac cgagccaaag cgctctttgc cttcgaggag
     6061 gtggatggca tcgatgtgtg cttctttggc atgcacgttc aggagtatgg atccgagtgc
     6121 ccggcgccga atacgcggcg tgtgtatatt gcctatttgg attccgttca tttcttccgg
     6181 ccaagacagt accgtacagc ggtatatcac gaaatcctgc tcggctatat ggactatgtg
     6241 aaacagctgg gctacacaat ggcccatatc tgggcctgtc cgccatccga gggcgatgac
     6301 tacatctttc actgccatcc cacggaccag aagataccca agcccaagcg cctgcaggag
     6361 tggtacaaaa agatgcttga caagggaatg atcgagcgca tcatacagga ctacaaggat
     6421 atcctgaagc aggcgatgga ggacaaactg ggctctgccg cagagctgcc ctactttgag
     6481 ggcgacttct ggcccaatgt gctggaggag agcatcaagg aactggacca ggaggaggaa
     6541 gagaagcgca aacaggccga ggccgcggaa gcagcagctg cggcaaatct tttctctatc
     6601 gaggaaaatg aagtaagcgg cgatggcaaa aagaagggcc agaagaaggc caaaaagtcg
     6661 aacaaatcga aagcggcgca gcgtaagaac agcaaaaagt ccaacgaaca tcagtcgggc
     6721 aatgatctct ccacaaagat atatgcgacc atggagaagc acaaggaggt cttcttcgtt
     6781 atccgtctgc attcggcgca gtcggcagct agtttagcgc ccatccagga tcccgatccg
     6841 ctgctcacat gcgatctgat ggatggacgc gatgccttcc tcacgctcgc ccgcgacaag
     6901 cactttgagt tctcgtcgct gcggcgcgca caattctcca ctctgtccat gttgtatgag
     6961 ctgcataacc agggtcagga caagtttgtt tacacctgca accactgcaa gacggccgtg
     7021 gagacgcgct accactgtac tgtttgtgat gacttcgatc tgtgtatcgt gtgcaaggag
     7081 aaggttggcc atcagcacaa gatggagaag ctcggcttcg acatcgacga cggctctgcg
     7141 ctggcggatc acaagcaggc taatccacag gaggcccgca agcaatccat ccagcgttgc
     7201 atccaatcgc tggcgcacgc ctgccagtgt cgcgatgcca actgccgcct gccatcgtgc
     7261 cagaagatga agctcgttgt ccagcatacg aagaactgca agcgcaagcc caacggagga
     7321 tgccccattt gcaagcagct tatcgcactc tgttgctatc acgcgaagaa ctgtgaggag
     7381 cagaagtgcc ccgtgccgtt ctgtcccaac atcaagcaca agctcaagca gcagcagtca
     7441 cagcagaaat tccagcagca gcagttgctg cgtcgccgtg tggcgctcat gtcgcgtaca
     7501 gcagctccag cggctctgca aggcccagct gcagtaagcg gtccgaccgt cgtctctgga
     7561 ggagtgcccg tggtgggcat gtccggtgtg gcagttagcc aacaggtgat ccccggccag
     7621 gcgggtatac tgcctccagg ggcgggtggc atgtcgccat ctaccgtggc agttccatcg
     7681 cctgtttcag gaggagcggg agccggtgga atgggtggaa tgacatcacc acatccgcat
     7741 caaccaggta taggtatgaa acctggtggc ggtcactcgc cgtctccaaa tgtcctacaa
     7801 gtggtgaagc aggtccagga agaggcagct cgtcagcagg tatcgcatgg cggtggcttc
     7861 ggcaagggcg tacccatggc gccgcccgta atgaatcgac caatgggcgg cgctgggccc
     7921 aaccaaaatg ttgttaatca acttggtggc atgggcgttg gagttggagg tgtcggtggt
     7981 gttggcgtcg gaggcgttgg tggagtgggt gttaatcaac tgaattcggg tggtggcaat
     8041 acacccggtg cacccatttc cggtcccgga atgaatgtca gtcatctaat gtccatggat
     8101 cagtggggcg gtggcggagc cggcggcgga ggtgccaatc ccggcggtgg caatccacaa
     8161 gcccgctatg ccaacaatac cggcggcatg cgccaaccca cccatgtgat gcaaacgaat
     8221 ctgataccgc cgcagcaaca gcaacagatg atgggcggac tgggcggacc caaccaactg
     8281 ggaggtggcc aaatgccagt cggcggacag catggaggaa tgggaatggg catgggagca
     8341 ccaccaatgg ccggaactgt tggcggagtg cgtccatctc ccggagcagg aggtggaggt
     8401 ggaagtgcga ctgggggcgg tctaaatacg caacaactcg ccctgattat gcaaaagatt
     8461 aagaacaatc ccaccaacga gagcaaccag cacatccttg ccatactaaa acagaatccg
     8521 cagatcatgg cggcgatcat caagcagcgc cagcagtcgc agaacaatgc ggcagcgggc
     8581 ggaggagcac ctggcccagg tggagcccta cagcagcagc aggccggtaa cggaccgcaa
     8641 aatcctcaac agcagcagca gcagcagcaa cagcaacagg tgatgcagca acagcagatg
     8701 cagcacatga tgaaccagca gcagggcggc ggcggtccac agcagatgaa tcccaaccag
     8761 cagcagcaac agcagcaggt taatctcatg cagcagcagc aacaaggtgg acccggagga
     8821 ccaggttctg gacttcccac gcgcatgccc aatatgccca atgccttggg tatgctgcag
     8881 agtcttccgc ccaacatgtc gccaggcgtt tctactcagg gaggaatggt gcccaaccaa
     8941 aactggaaca agatgcgtta catgcaaatg agccagtacc cgccaccgta tccgcagcgc
     9001 cagcgtggcc cgcacatggg cggagcggga cctggtcccg gccagcaaca gttccccggt
     9061 ggcggaggtg gagcgggcaa ctttaatgcg ggtggtgctg gtggtgcagg cggcgttgtc
     9121 ggtgtgggcg gagtgcccgg aggtgccggc acggtgcccg gtggcgatca atactcgatg
     9181 gcgaatgccg cggctgcctc caatatgctg caacagcagc agggccaggt gggcgtcgga
     9241 gtgggcgtgg gcgtgaaacc aggacccggc caacagcaac agcagatggg cgttggcatg
     9301 ccgccgggta tgcagcagca acagcagcaa cagcaaccgc tgcagcagca gcagatgatg
     9361 caggtagcaa tgccaaatgc gaatgcccag aatccgtcgg cggtggttgg cggacccaat
     9421 gctcaggtga tgggtccgcc gacgccgcac tctctgcagc agcagctgat gcaatcggcc
     9481 cgctcgtcgc cgcctattcg ctccccgcag ccaacgccat cgccacgttc ggctccatcg
     9541 ccacgtgctg ctccatccgc ctcgcctagg gcacagccct cgccgcacca tgtgatgagc
     9601 agtcactcgc cagcgccgca gggaccaccg catgacggca tgcacaatca tggcatgcat
     9661 catcagtcgc cactgccagg agtgccgcag gatgttggcg tcggagtcgg tgtcggcgtt
     9721 ggcgttggcg ttaacgttaa cgtcggcaac gtgggcgtcg gcaatgccgg aggagccctg
     9781 cccgacgcct ccgaccagct gaccaagttt gtggagcgac tc
//
