LOCUS       sequence-138956-        7907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Sema3B protein (truncated at cleavage site P3; ie, 
            long), C-terminally fused to the Fc region of human IgG1 + 6X 
            histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7907)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 7907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema3b(L)-Fc-His
FEATURES             Location/Qualifiers
     source          1..7907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4487..4504
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4530..4651
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4567..4586)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4621..4640
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4689..4706)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4860..5448)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4940..4959)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5619..6410,6411..6479))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6242..6261
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6480..6584)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6652..6670
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6708..6730)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6732..6752)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6737..7067
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6918..7053
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6980..6999
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7204..7223
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7290..7745)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7427..7448)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7639..7658
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7871..7893
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7885..7902
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7886..7902
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggggcgggc
     1621 tgaggccgcc gccatgatcc caggcctggc ccttctctgg gtagcagggc taggggatac
     1681 tgcccctaac cttccccgcc ttcggctctc ctttcaagaa ttacaggccc ggcatggtgt
     1741 ccgaaccttc aggctggagc ggacctgctg ttatgaagcc ttgctggtgg atgaggagcg
     1801 tggacgcctg tttgtgggtg ctgagaacca cgtggcttcc ctcagcctgg acaacatcag
     1861 caagcgagcc aagaagttgg cctggcccgc ccccgtggaa tggcgtgaag aatgcaactg
     1921 ggcagggaag gacattggta ccgagtgcat gaacttcatg aagctgctgc acgcctacaa
     1981 ccacacccac ttgctggcct gtggcacagg ggctttccac ccaacctgcg cctttgtgga
     2041 ggtgggccac cggctggagg aacccatgct tcaactggac cggaggaaac ttgaggacgg
     2101 caaggggaag actccttatg acccaaggca tcgggctgcc tcggtgctgg tgggggaaga
     2161 gctgtattct ggggtggcag cagaccttat gggccgggac tttaccatct ttcgaagcct
     2221 tggtcagaat ccgagtctcc gaacagagcc ccatgattcc cgctggctca atgaacccaa
     2281 gtttgtcaag gtcttttgga tcccagagag tgagaaccct gatgacgata aaatctattt
     2341 cttcttccgc gagtccgctg tggaagcagc accagcaatg gggcgcatgt ctgtgtctcg
     2401 tgttggccag atctgcagga atgacctggg tggccagcgg agcttggtca acaaatggac
     2461 cacatttctg aaggcgcggc ttgtgtgctc agtacctgga gttgagggtg acacccactt
     2521 tgaccaactt caggatgttt tccttctgtc ctcccgagac cgccagacac ctcttctcta
     2581 tgctgtcttc tccacctcca gtggtgtctt ccagggctct gctgtgtgcg tgtacagcat
     2641 gaacgatgtg cgccgagcct tcttgggacc ttttgctcac aaagaggggc ctacacacca
     2701 gtgggtgtcc taccagggtc gtgtccccta cccaagacct ggcatgtgcc ccagcaagac
     2761 ctttggcacc ttcagctcca ccaaggactt cccagatgac gttatccagt ttgctcggaa
     2821 ccaccctctc atgtacaacc cagtcctgcc catggggggg cgccctctct tcctacaagt
     2881 gggagctggg tacaccttca cccaaatcgc cgcagaccga gtagcagctg ccgatggaca
     2941 ctacgatgtt ctcttcattg gtacagatgt gggcacagtg ctgaaagtga tctcagtccc
     3001 caaaggcagc cgacctaatt ctgaaggact tctcctggaa gagctgcagg tgttcgagga
     3061 ctctgccgct atcaccagca tgcaaatctc ctctaaaagg caacaactct acgtagcatc
     3121 gcgcagcgca gtggcccaga ttgctttgca tcgctgcact gccctaggcc gcgcctgcgc
     3181 agaatgctgc ttggcccgtg atccttactg cgcctgggat ggatcagctt gcacacgctt
     3241 ccagcctacg gccaagagac ggttccggag gcaagacata aggaatggcg accccagcac
     3301 cctatgctct ggagactctt ctcactctgt gctgctggag aagaaggtgt tgggtgtgga
     3361 gagcggcagc gcgtttctgg agtgtgagcc ccgctcgctc caggcgcatg tgcagtggac
     3421 cttccaaggt gcaggggagg cagctcacac ccaggtgctg gctgaggaga gagtagagcg
     3481 cactgcgcgg gggctgctgt tgcgggggct gcggcgccag gattctggcg tgtatctatg
     3541 cgtcgcggtt gaacaaggct tttcacaacc actgcgtcgc ctggtgctgc atgtgttgag
     3601 tgcggcgcag gctgaacgac tggcacgggc agaggaagca gccgctcctg cacctcctgg
     3661 ccctaaactc tggtaccggg actttctgca gttggtggag ccaggcggtg gcggaggtgc
     3721 aaactccctg cgaatgtgcc gcccgcagcc cgggcaccat tctgtggcag cagattcacg
     3781 tcgtaagggt ggcgcgcctg acaaaactca cacatgccca ccgtgcccag cacctgaact
     3841 cctgggggga ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc
     3901 ccggacccct gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa
     3961 gttcaactgg tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga
     4021 gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct
     4081 gaatggcaag gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa
     4141 aaccatctcc aaagccaaag ggcagccccg agaaccacag gtgtataccc tgcccccatc
     4201 ccgggatgag ctgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc
     4261 cagcgacatc gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac
     4321 gcctcccgtg ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa
     4381 gagcaggtgg cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa
     4441 ccactacacg cagaagagcc tctccctgtc tccgggtaaa ggcggccatc atcaccatca
     4501 ccattaatct agagatctta ttaaagcaga acttgtttat tgcagcttat aatggttaca
     4561 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4621 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gtcgactcta gctagactct
     4681 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4741 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     4801 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4861 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4921 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4981 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5041 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5101 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5161 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5221 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5281 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5341 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5401 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5461 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     5521 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     5581 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     5641 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     5701 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     5761 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     5821 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     5881 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     5941 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6001 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6061 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6121 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     6181 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     6241 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     6301 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     6361 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     6421 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     6481 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     6541 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     6601 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     6661 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     6721 cagctcccgg aggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag
     6781 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     6841 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     6901 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6961 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     7021 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     7081 cttgtatatc cattttcgtc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     7141 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     7201 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     7261 gatgcgtaag gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt
     7321 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     7381 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     7441 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     7501 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     7561 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     7621 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     7681 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     7741 cgcgtccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     7801 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     7861 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaatt
//
