LOCUS       sequence-138965-        7502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Sema6A, isoform z protein,
            C-terminally fused to the Fc region of human IgG1 + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7502)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 7502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema6a.z-Fc-His
FEATURES             Location/Qualifiers
     source          1..7502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             4082..4099
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4125..4246
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4162..4181)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4216..4235
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4284..4301)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4455..5043)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4535..4554)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5214..6005,6006..6074))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5837..5856
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6075..6179)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6247..6265
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6303..6325)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6327..6347)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6332..6662
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6513..6648
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6575..6594
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6799..6818
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6885..7340)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7022..7043)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7234..7253
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7466..7488
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7480..7497
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7481..7497
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgcggccagc
     1621 agccttactg ctgtgtctca cactgctaca ctgcgctggg gctggtttcc cagaagattc
     1681 cgagccaatc agtatttcgc atggcaacta tacaaaacag tatccggtgt ttgtgggcca
     1741 caagccagga cggaacacca cgcagaggca caggctggac atccagatga tcatgatcat
     1801 gaacagaacc ctctacgttg ctgctcgaga ccatatttat actgttgata tagacacatc
     1861 ccacacagaa gaaatttact gtagcaaaaa actgacatgg aaatctagac aggctgacgt
     1921 agacacatgc aggatgaagg ggaaacataa ggatgaatgt cacaacttca ttaaagttct
     1981 tctcaagaag aatgatgata cgctgtttgt ctgtggaacc aatgccttca acccttcctg
     2041 cagaaactac agggtcgata ccttggaaac ttttggggat gaatttagcg gaatggccag
     2101 atgcccttat gatgccaaac atgccaacat cgctctgttt gcagatggaa aactctactc
     2161 ggctacagtg actgactttc tggccattga tgcggtcatt tacaggagcc tcggagacag
     2221 ccctaccctc aggactgtca agcatgattc aaagtggttg aaagagccgt actttgtcca
     2281 agccgtggat tatggggact atatctactt cttcttcaga gaaattgcag tagaatacaa
     2341 cactatgggg aaggttgttt tccctagggt ggctcaggtc tgtaagaatg acatgggagg
     2401 gtctcagaga gtcctggaga agcagtggac atctttcctg aaggctcgcc tgaactgctc
     2461 ggtgcctgga gactctcatt tttatttcaa tatactccag gcagttacag atgtgattcg
     2521 cattaatggc cgtgatgttg tcttggcaac cttttccaca ccttataaca gcatcccagg
     2581 ttctgcagtc tgtgcctatg acatgcttga cattgctaat gttttcactg ggaggttcaa
     2641 ggaacagaaa tcacctgact ctacctggac acccgttcca gacgaacgag tccctaagcc
     2701 caggccaggc tgttgtgctg gatcatcctc tttagaaaaa tatgcaacct ccaatgagtt
     2761 tcccgatgat accctgaact tcattaagac gcatccactc atggacgagg cagtgccttc
     2821 catcatcaac agaccttggt tcctgagaac aatggtcaga taccgcctga ccaaaattgc
     2881 agtagacaac gctgccgggc catatcagaa tcacactgtg gttttcctgg gatcagaaaa
     2941 gggaatcatc ctgaagttct tggccaggat aggaagcagt ggtttcctaa atggcagcct
     3001 tttcctggag gagatgaatg tttacaaccc agaaaagtgc agctatgatg gtgtagaaga
     3061 caaaaggatc atgggcatgc agctcgacag agcgagtggc tcactctatg ttgcattctc
     3121 tacttgtgtg atcaaggtgc ctcttggccg ctgtgagcga catgggaagt gtaaaaaaac
     3181 ctgcatcgcc tccagagacc cgtattgtgg gtgggtaagg gaaagtggtt cctgtgccca
     3241 tctgtcaccc cttagcagac tgacatttga gcaggacatt gagcgtggca atacggacgg
     3301 cctaggagac tgtcacaatt ccttcgtggc actgaatgga gtgattcggg aaagttacct
     3361 caaaagcaac gaccagcttg ttcctggcgc gcctgacaaa actcacacat gcccaccgtg
     3421 cccagcacct gaactcctgg ggggaccgtc agtcttcctc ttccccccaa aacccaagga
     3481 caccctcatg atctcccgga cccctgaggt cacatgcgtg gtggtggacg tgagccacga
     3541 agaccctgag gtcaagttca actggtacgt ggacggcgtg gaggtgcata atgccaagac
     3601 aaagccgcgg gaggagcagt acaacagcac gtaccgtgtg gtcagcgtcc tcaccgtcct
     3661 gcaccaggac tggctgaatg gcaaggagta caagtgcaag gtctccaaca aagccctccc
     3721 agcccccatc gagaaaacca tctccaaagc caaagggcag ccccgagaac cacaggtgta
     3781 taccctgccc ccatcccggg atgagctgac caagaaccag gtcagcctga cctgcctggt
     3841 caaaggcttc tatcccagcg acatcgccgt ggagtgggag agcaatgggc agccggagaa
     3901 caactacaag accacgcctc ccgtgctgga ctccgacggc tccttcttcc tctacagcaa
     3961 gctcaccgtg gacaagagca ggtggcagca ggggaacgtc ttctcatgct ccgtgatgca
     4021 tgaggctctg cacaaccact acacgcagaa gagcctctcc ctgtctccgg gtaaaggcgg
     4081 ccatcatcac catcaccatt aatctagaga tcttattaaa gcagaacttg tttattgcag
     4141 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     4201 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggtcga
     4261 ctctagctag actcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     4321 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4381 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4441 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4501 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4561 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4621 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4681 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4741 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4801 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4861 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     4921 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4981 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5041 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5101 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5161 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5221 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5281 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5341 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5401 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5461 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5521 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5581 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5641 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5701 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5761 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5821 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5881 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5941 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     6001 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     6061 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6121 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6181 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     6241 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     6301 aacctctgac acatgcagct cccggaggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     6361 aggctcccca ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     6421 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6481 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     6541 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     6601 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     6661 aaaagctccc gggagcttgt atatccattt tcgtcccgga gacggtcaca gcttgtctgt
     6721 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     6781 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     6841 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg taagcgttaa
     6901 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     6961 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     7021 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     7081 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     7141 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     7201 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     7261 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     7321 tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     7381 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     7441 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     7501 tt
//
