LOCUS       sequence-138966-        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express an NT-3-BDNF hybrid neurotrophic factor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Enomoto M, Bunge MB, Tsoulfas P
  TITLE     A multifunctional neurotrophin with reduced affinity to p75NTR 
            enhances transplanted Schwann cell survival and axon growth after 
            spinal cord injury.
  JOURNAL   Exp Neurol. 2013 Oct;248:170-82. doi: 
            10.1016/j.expneurol.2013.06.013. Epub 2013 Jun 20.
  PUBMED    23792206
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Neurol."; date: "2013-10"; volume: "248:170-82. doi"; pages: " 
            10.1016/j.expneurol.2013.06.013. Epub 2013 Jun 20"
COMMENT     Sequence Label: pLV-NT-3/D15A
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(957..977)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgactcta gaggatccta caggtgaaca agaccatgtc
      121 catcttgttt tatgtgatat ttctcgctta tctccgtggc atccaaggta acaacatgga
      181 tcaaaggagt ttgccagaag actcgctcaa ttccctcatt attaagctga tccaggcaga
      241 tattttgaaa aacaagctct ccaagcagat ggtggacgtt aaggaaaatt accagagcac
      301 cctgcccaaa gctgaggctc cccgagagcc ggagcgggga gggcccgcca agtcagcatt
      361 ccagccggtg attgcaatgg acaccgaact gctgcgacaa cagagacgct acaactcacc
      421 gcgggtcctg ctgagcgaca gcaccccctt ggagcccccg cccttgtatc tcatggagga
      481 ttacgtgggc agccccgtgg tggcgaacag aacatcacgg cggaaacggt acgcggagca
      541 taagagtcac cgaggggagt actcggtatg tgccagtgaa agcttgtggg tgaccgacaa
      601 gtcatcggcc atcgacattc ggggacacca ggtcacggtg ctgggggaga tcaaaacggg
      661 caactctccc gtcaaacaat atttttatga aacgcgatgt aaggaagcca ggccggtcaa
      721 aaacggttgc aggggtattg atgataaaca ctggaactct cagtgcaaaa catcccaaac
      781 ctacgtccga gcactgactt cagagaacaa taaactcgtg ggctggcggt ggatacggat
      841 agacacgtcc tgtgtgtgtg ccttgtcgag aaaaatcgga agaacatgaa ttggcatctc
      901 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
      961 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     1021 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     1081 ttgtgg
//
