LOCUS       sequence-138967-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express an NT-3-BDNF hybrid neurotrophic factor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Enomoto M, Bunge MB, Tsoulfas P
  TITLE     A multifunctional neurotrophin with reduced affinity to p75NTR 
            enhances transplanted Schwann cell survival and axon growth after 
            spinal cord injury.
  JOURNAL   Exp Neurol. 2013 Oct;248:170-82. doi: 
            10.1016/j.expneurol.2013.06.013. Epub 2013 Jun 20.
  PUBMED    23792206
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Neurol."; date: "2013-10"; volume: "248:170-82. doi"; pages: " 
            10.1016/j.expneurol.2013.06.013. Epub 2013 Jun 20"
COMMENT     Sequence Label: pLV-NT-3/D15A
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(931..955)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(952..1003)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(981..1001)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aaggagcaac atagttaaga ataccagtca atctttcaca aattttgtaa tccagaggtt
       61 gattgtcgag atgccaattc atgttcttcc gatttttctc gacaaggcac acacacagga
      121 cgtgtctatc cgtatccacc gccagcccac gagtttattg ttctctgaag tcagtgctcg
      181 gacgtaggtt tgggatgttt tgcactgaga gttccagtgt ttatcatcaa tacccctgca
      241 accgtttttg accggcctgg cttccttaca tcgcgtttca taaaaatatt gtttgacggg
      301 agagttgccc gttttgatct cccccagcac cgtgacctgg tgtccccgaa tgtcgatggc
      361 cgatgacttg tcggtcaccc acaagctttc actggcacat accgagtact cccctcggtg
      421 actcttatgc tccgcgtacc gtttccgccg tgatgttctg ttcgccacca cggggctgcc
      481 cacgtaatcc tccatgagat acaagggcgg gggctccaag ggggtgctgt cgctcagcag
      541 gacccgcggt gagttgtagc gtctctgttg tcgcagcagt tcggtgtcca ttgcaatcac
      601 cggctggaat gctgacttgg cgggccctcc ccgctccggc tctcggggag cctcagcttt
      661 gggcagggtg ctctggtaat tttccttaac gtccaccatc tgcttggaga gcttgttttt
      721 caaaatatct gcctggatca gcttaataat gagggaattg agcgagtctt ctggcaaact
      781 cctttgatcc atgttgttac cttggatgcc acggagataa gcgagaaata tcacataaaa
      841 caagatggac atggtcttgt tcacctgtag gatcctctag agtcggtgtc ttctatggag
      901 gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac gagctctgct
      961 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cgga
//
