LOCUS       sequence-138976-        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PSD-95 tagged with mCherry. Expression is Cre dependent. 
            Plasmid can also be used to make lentivirus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Villa KL, Berry KP, Subramanian J, Cha JW, Oh WC, Kwon HB, Kubota Y,
            So PT, Nedivi E
  TITLE     Inhibitory Synapses Are Repeatedly Assembled and Removed at 
            Persistent Sites In Vivo.
  JOURNAL   Neuron. 2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 
            10.1016/j.neuron.2016.01.010.
  PUBMED    26853302
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 
            10.1016/j.neuron.2016.01.010."
COMMENT     Sequence Label: FU- dio PSD95-mCherry- W
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(178..211)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(262..295)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(316..1026)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(594..613)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     863..881
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gtgtgttttg tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta
       61 attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt
      121 tagacgaagc ttgggctgca ggtcgactct agaggatcct ctagagtcga ctccggaata
      181 acttcgtata ggatacttta tacgaagtta tgcagaatgg tagctggatt gtagctgcta
      241 ttagcaatat gaaacctctt aataacttcg tatagcatac attatacgaa gttatggcgc
      301 gccttgacga ccggtttact tgtacagctc gtccatgccg ccggtggagt ggcggccctc
      361 ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccaactt
      421 gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt aggtggtctt
      481 gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga tctcgccctt
      541 cagggcgccg tcctcggggt acatccgctc ggaggaggcc tcccagccca tggtcttctt
      601 ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa
      661 ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt
      721 catcacgcgc tcccacttga agccctcggg gaaggacagc ttcaagtagt cggggatgtc
      781 ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac tgaggggaca ggatgtccca
      841 ggcgaagggc agggggccac ccttggtcac cttcagcttg gcggtctggg tgccctcgta
      901 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat
      961 gtgcaccttg aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct
     1021 caccatgaat tcgagtctc
//
