LOCUS       sequence-138978-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible sucrose counter-selection allelic exchange vector
            for Pseudomonas syringae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  TITLE     Kvitko lab Gateway compatible allelic exchange plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDONR2T18ms
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(423..442)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct aacgcagtca
       61 ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga
      121 tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca
      181 ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt
      241 tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct
      301 cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg
      361 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
      421 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
      481 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
      541 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
      601 ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt
      661 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
      721 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg
      781 cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat
      841 cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa
      901 gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc
      961 gacgcgaggc tgg
//
