LOCUS       sequence-138983-        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PSD-95 tagged with mCherry. Expression is Cre dependent. 
            Plasmid can also be used to make lentivirus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Villa KL, Berry KP, Subramanian J, Cha JW, Oh WC, Kwon HB, Kubota Y,
            So PT, Nedivi E
  TITLE     Inhibitory Synapses Are Repeatedly Assembled and Removed at 
            Persistent Sites In Vivo.
  JOURNAL   Neuron. 2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 
            10.1016/j.neuron.2016.01.010.
  PUBMED    26853302
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 
            10.1016/j.neuron.2016.01.010."
COMMENT     Sequence Label: FU- dio PSD95-mCherry- W
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(63..96)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(147..180)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      188..197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             230..259
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat
       61 tcataacttc gtatagcata cattatacga agttattctt tgcaccattc taaagaataa
      121 cagtgataat ttctgggtta aggcaaataa cttcgtatag gatactttat acgaagttat
      181 gctagccgcc accatggact gtctctgtat agtgacaacc cggcgcgccg agcaaaagct
      241 gatatctgaa gaggacttgg ggcgcgccaa gaaataccgc taccaagatg aagacacgcc
      301 ccctctggaa cacagcccgg cccacctccc caaccaggcc aattctcccc ctgtgattgt
      361 caacacggac accctagaag ccccaggata tgagttgcag gtgaatggaa cagaggggga
      421 gatggagtat gaggagatca cattggaaag gggtaactca ggtctgggct tcagcatcgc
      481 aggtggcact gacaacccgc acatcggtga cgacccgtcc atttttatca ccaagatcat
      541 tcctggtggg gctgcagccc aggatggccg cctcagggtc aatgacagca tcctgtttgt
      601 aaatgaagtg gatgttcggg aggtgaccca ttcagctgcg gtggaggccc tcaaagaggc
      661 aggttccatc gttcgccact atgtcatgcg ccggaaaccc ccagccgaaa aggtcatgga
      721 gatcaaactc atcaaagggc ctaaaggact tggcttcagc attgcggggg gcgttgggaa
      781 ccagcacatc cctggagata acagcatcta tgtaacgaag atcatcgaag gaggtgctgc
      841 tcacaaggat ggcaggttgc agattggaga caagatcctg gcggtcaaca gtgtggggct
      901 ggaggacgtt atgcacgagg atgccgtggc agccctgaag aacacatatg acgttgtgta
      961 cctaaaggtg gccaagccca gcaatgccta cctgagtgac agctatgctc ccc
//
